Detailed information of TA system TA199950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4718167..4718683
• Replicon: chromosome
• Accession: NZ_CP073627
• Organism: Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: KD921_RS22825
• Protein ID: WP_004178374.1
• Coordinates: 4718167..4718451 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: KD921_RS22830
• Protein ID: WP_032434351.1
• Coordinates: 4718441..4718683 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD921_RS22800 (4713650)	4713650..4713913	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
KD921_RS22805 (4714043)	4714043..4714216	+	174	WP_032414379.1	hypothetical protein	-
KD921_RS22810 (4714219)	4714219..4714962	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
KD921_RS22815 (4715319)	4715319..4717457	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
KD921_RS22820 (4717699)	4717699..4718163	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KD921_RS22825 (4718167)	4718167..4718451	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KD921_RS22830 (4718441)	4718441..4718683	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KD921_RS22835 (4718761)	4718761..4720671	-	1911	WP_004152270.1	PRD domain-containing protein	-
KD921_RS22840 (4720694)	4720694..4721848	-	1155	WP_002886900.1	lactonase family protein	-
KD921_RS22845 (4721914)	4721914..4722654	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T199950 WP_004178374.1 NZ_CP073627:c4718451-4718167 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T199950 NZ_CP097666:c54492-54187 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT199950 WP_032434351.1 NZ_CP073627:c4718683-4718441 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT199950 NZ_CP097666:c54712-54494 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A6THG1