Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4594775..4595585 Replicon chromosome
Accession NZ_CP073627
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KD921_RS22300 Protein ID WP_004178461.1
Coordinates 4594775..4595308 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KD921_RS22305 Protein ID WP_002887278.1
Coordinates 4595319..4595585 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD921_RS22295 (4593606) 4593606..4594727 + 1122 WP_004186850.1 cupin domain-containing protein -
KD921_RS22300 (4594775) 4594775..4595308 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KD921_RS22305 (4595319) 4595319..4595585 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KD921_RS22310 (4595688) 4595688..4597121 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
KD921_RS22315 (4597111) 4597111..4597794 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KD921_RS22320 (4597967) 4597967..4599352 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
KD921_RS22325 (4599370) 4599370..4599714 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T199948 WP_004178461.1 NZ_CP073627:c4595308-4594775 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T199948 NZ_CP097665:124219-124542 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAGCTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACAATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GGCGCCCGGGGATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT199948 WP_002887278.1 NZ_CP073627:c4595585-4595319 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT199948 NZ_CP097665:124591-124893 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTACACTGATTGATACGTTGCGTGGTATTCACGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References