Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3953067..3953686 Replicon chromosome
Accession NZ_CP073627
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KD921_RS19220 Protein ID WP_002892050.1
Coordinates 3953468..3953686 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KD921_RS19215 Protein ID WP_002892066.1
Coordinates 3953067..3953441 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD921_RS19205 (3948219) 3948219..3949412 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KD921_RS19210 (3949435) 3949435..3952581 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KD921_RS19215 (3953067) 3953067..3953441 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KD921_RS19220 (3953468) 3953468..3953686 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KD921_RS19225 (3953845) 3953845..3954411 + 567 WP_002892030.1 maltose O-acetyltransferase -
KD921_RS19230 (3954383) 3954383..3954523 - 141 WP_004147370.1 hypothetical protein -
KD921_RS19235 (3954544) 3954544..3955014 + 471 WP_002892026.1 YlaC family protein -
KD921_RS19240 (3954989) 3954989..3956440 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
KD921_RS19245 (3956541) 3956541..3957239 + 699 WP_032435564.1 GNAT family protein -
KD921_RS19250 (3957236) 3957236..3957376 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KD921_RS19255 (3957376) 3957376..3957639 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199947 WP_002892050.1 NZ_CP073627:3953468-3953686 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T199947 NZ_CP097665:c20933-20517 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCACTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT199947 WP_002892066.1 NZ_CP073627:3953067-3953441 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT199947 NZ_CP097665:c21160-20930 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References