Detailed information of TA system TA199930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4264123..4264718
• Replicon: chromosome
• Accession: NZ_CP073624
• Organism: Escherichia coli strain AW1.7

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: KD735_RS20865
• Protein ID: WP_000239581.1
• Coordinates: 4264123..4264473 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: KD735_RS20870
• Protein ID: WP_001223213.1
• Coordinates: 4264467..4264718 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD735_RS20845 (4259572)	4259572..4260594	-	1023	WP_001548970.1	ABC transporter permease	-
KD735_RS20850 (4260608)	4260608..4262110	-	1503	WP_001095667.1	sugar ABC transporter ATP-binding protein	-
KD735_RS20855 (4262248)	4262248..4263204	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
KD735_RS20860 (4263514)	4263514..4264044	+	531	WP_000055072.1	inorganic diphosphatase	-
KD735_RS20865 (4264123)	4264123..4264473	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
KD735_RS20870 (4264467)	4264467..4264718	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
KD735_RS20875 (4264930)	4264930..4265271	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
KD735_RS20880 (4265274)	4265274..4269053	-	3780	WP_054625935.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T199930 WP_000239581.1 NZ_CP073624:c4264473-4264123 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T199930 NZ_CP097660:c4923951-4923667 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT199930 WP_001223213.1 NZ_CP073624:c4264718-4264467 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT199930 NZ_CP097660:c4924183-4923941 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7