Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4086238..4086788 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag KD735_RS19985 Protein ID WP_001160971.1
Coordinates 4086238..4086552 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KD735_RS19990 Protein ID WP_000125566.1
Coordinates 4086555..4086788 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS19960 (4081913) 4081913..4083199 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KD735_RS19965 (4083199) 4083199..4084188 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KD735_RS19970 (4084185) 4084185..4085006 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KD735_RS19975 (4085009) 4085009..4085386 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KD735_RS19980 (4085393) 4085393..4086241 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KD735_RS19985 (4086238) 4086238..4086552 - 315 WP_001160971.1 CcdB family protein Toxin
KD735_RS19990 (4086555) 4086555..4086788 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KD735_RS19995 (4086874) 4086874..4087353 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
KD735_RS20000 (4087545) 4087545..4089407 - 1863 WP_001412464.1 glutathione-regulated potassium-efflux system protein KefC -
KD735_RS20005 (4089400) 4089400..4089930 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KD735_RS20010 (4090041) 4090041..4091372 - 1332 WP_001183198.1 MFS transporter -
KD735_RS20015 (4091430) 4091430..4091717 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T199928 WP_001160971.1 NZ_CP073624:c4086552-4086238 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T199928 NZ_CP097660:4208455-4208673 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT199928 WP_000125566.1 NZ_CP073624:c4086788-4086555 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT199928 NZ_CP097660:4208054-4208428 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References