Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3897115..3897902 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2H9G3C1
Locus tag KD735_RS19115 Protein ID WP_001568519.1
Coordinates 3897525..3897902 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag KD735_RS19110 Protein ID WP_000066236.1
Coordinates 3897115..3897474 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS19070 (3892811) 3892811..3892957 + 147 WP_023563913.1 hypothetical protein -
KD735_RS19075 (3892970) 3892970..3893185 + 216 WP_041036768.1 hypothetical protein -
KD735_RS19080 (3893299) 3893299..3893709 + 411 WP_042851426.1 hypothetical protein -
KD735_RS19085 (3893883) 3893883..3894713 + 831 WP_225858599.1 hypothetical protein -
KD735_RS19090 (3894802) 3894802..3895620 + 819 WP_016236913.1 DUF932 domain-containing protein -
KD735_RS19095 (3895888) 3895888..3896358 + 471 WP_000131762.1 antirestriction protein -
KD735_RS19100 (3896370) 3896370..3896849 + 480 WP_000437750.1 DNA repair protein RadC -
KD735_RS19105 (3896870) 3896870..3897091 + 222 WP_000691981.1 DUF987 domain-containing protein -
KD735_RS19110 (3897115) 3897115..3897474 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KD735_RS19115 (3897525) 3897525..3897902 + 378 WP_001568519.1 TA system toxin CbtA family protein Toxin
KD735_RS19120 (3897899) 3897899..3898390 + 492 WP_000777682.1 DUF5983 family protein -
KD735_RS19125 (3898422) 3898422..3898625 + 204 WP_000413747.1 DUF957 domain-containing protein -
KD735_RS19130 (3898706) 3898706..3899551 + 846 WP_016236912.1 DUF4942 domain-containing protein -
KD735_RS19140 (3899893) 3899893..3900624 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
KD735_RS19145 (3900689) 3900689..3901156 + 468 WP_000917883.1 ribonuclease HI -
KD735_RS19150 (3901153) 3901153..3901875 - 723 WP_001295200.1 class I SAM-dependent methyltransferase -
KD735_RS19155 (3901909) 3901909..3902664 + 756 WP_001052721.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3876088..3907745 31657


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14211.00 Da        Isoelectric Point: 7.4209

>T199927 WP_001568519.1 NZ_CP073624:3897525-3897902 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKTTGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T199927 NZ_CP097660:c2745433-2744111 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT199927 WP_000066236.1 NZ_CP073624:3897115-3897474 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT199927 NZ_CP097660:c2745699-2745433 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9G3C1

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References