Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3826745..3827424 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag KD735_RS18725 Protein ID WP_008324514.1
Coordinates 3827083..3827424 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag KD735_RS18720 Protein ID WP_008324516.1
Coordinates 3826745..3827062 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS18675 (3822186) 3822186..3823007 + 822 WP_054626066.1 DUF932 domain-containing protein -
KD735_RS18680 (3823225) 3823225..3823926 + 702 WP_054626065.1 WYL domain-containing protein -
KD735_RS18685 (3823963) 3823963..3824199 + 237 WP_001115854.1 protein YpjK -
KD735_RS18690 (3824199) 3824199..3824642 + 444 WP_008324520.1 lipoprotein YafY -
KD735_RS18695 (3824665) 3824665..3825132 + 468 WP_001547765.1 protein YkfB -
KD735_RS18700 (3825209) 3825209..3825448 + 240 WP_000194654.1 DUF905 family protein -
KD735_RS18705 (3825546) 3825546..3826004 + 459 WP_000211838.1 antirestriction protein -
KD735_RS18710 (3826020) 3826020..3826496 + 477 WP_000811693.1 RadC family protein -
KD735_RS18715 (3826505) 3826505..3826732 + 228 WP_008324517.1 DUF987 domain-containing protein -
KD735_RS18720 (3826745) 3826745..3827062 + 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KD735_RS18725 (3827083) 3827083..3827424 + 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
KD735_RS18735 (3827996) 3827996..3829249 - 1254 WP_000893260.1 glutamate-5-semialdehyde dehydrogenase -
KD735_RS18740 (3829261) 3829261..3830364 - 1104 WP_001285288.1 glutamate 5-kinase -
KD735_RS18745 (3830652) 3830652..3831707 + 1056 WP_000749881.1 phosphoporin PhoE -
KD735_RS18750 (3831746) 3831746..3832147 - 402 WP_000174677.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3818538..3827644 9106


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T199926 WP_008324514.1 NZ_CP073624:3827083-3827424 [Escherichia coli]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T199926 NZ_CP097660:2011052-2011154 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT199926 WP_008324516.1 NZ_CP073624:3826745-3827062 [Escherichia coli]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT199926 NZ_CP097660:c2011161-2011016 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References