Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3596794..3597412 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KD735_RS17620 Protein ID WP_001291435.1
Coordinates 3597194..3597412 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KD735_RS17615 Protein ID WP_000344800.1
Coordinates 3596794..3597168 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS17605 (3591883) 3591883..3593076 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KD735_RS17610 (3593099) 3593099..3596248 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KD735_RS17615 (3596794) 3596794..3597168 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KD735_RS17620 (3597194) 3597194..3597412 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KD735_RS17625 (3597584) 3597584..3598135 + 552 WP_000102564.1 maltose O-acetyltransferase -
KD735_RS17630 (3598251) 3598251..3598721 + 471 WP_000136192.1 YlaC family protein -
KD735_RS17635 (3598885) 3598885..3600435 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KD735_RS17640 (3600477) 3600477..3600830 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KD735_RS17650 (3601209) 3601209..3601520 + 312 WP_000409911.1 MGMT family protein -
KD735_RS17655 (3601551) 3601551..3602123 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199925 WP_001291435.1 NZ_CP073624:3597194-3597412 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T199925 NZ_CP097660:861765-862172 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT199925 WP_000344800.1 NZ_CP073624:3596794-3597168 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT199925 NZ_CP097660:861518-861784 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References