Detailed information of TA system TA199924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2591807..2592445
• Replicon: chromosome
• Accession: NZ_CP073624
• Organism: Escherichia coli strain AW1.7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KD735_RS12785
• Protein ID: WP_000813794.1
• Coordinates: 2592269..2592445 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KD735_RS12780
• Protein ID: WP_001270286.1
• Coordinates: 2591807..2592223 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD735_RS12760 (2586959)	2586959..2587900	-	942	WP_001251313.1	ABC transporter permease	-
KD735_RS12765 (2587901)	2587901..2588914	-	1014	WP_000220434.1	ABC transporter ATP-binding protein	-
KD735_RS12770 (2588932)	2588932..2590077	-	1146	WP_000047430.1	ABC transporter substrate-binding protein	-
KD735_RS12775 (2590322)	2590322..2591728	-	1407	WP_000760630.1	PLP-dependent aminotransferase family protein	-
KD735_RS12780 (2591807)	2591807..2592223	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KD735_RS12785 (2592269)	2592269..2592445	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KD735_RS12790 (2592667)	2592667..2592897	+	231	WP_072188679.1	YncJ family protein	-
KD735_RS12795 (2592989)	2592989..2594950	-	1962	WP_001488038.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KD735_RS12800 (2595023)	2595023..2595559	-	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
KD735_RS12805 (2595651)	2595651..2596826	+	1176	WP_001236305.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T199924 WP_000813794.1 NZ_CP073624:c2592445-2592269 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T199924 NZ_CP097660:727483-727968 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT199924 WP_001270286.1 NZ_CP073624:c2592223-2591807 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT199924 NZ_CP097660:727193-727486 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• PDB: 6HPB