Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 912211..912865 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KD735_RS04440 Protein ID WP_000244781.1
Coordinates 912458..912865 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KD735_RS04435 Protein ID WP_000354046.1
Coordinates 912211..912477 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS04410 (907376) 907376..908118 + 743 Protein_864 SDR family oxidoreductase -
KD735_RS04415 (908180) 908180..909613 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
KD735_RS04420 (909658) 909658..909969 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KD735_RS04425 (910133) 910133..910792 + 660 WP_000250274.1 hemolysin III family protein -
KD735_RS04430 (910988) 910988..911968 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
KD735_RS04435 (912211) 912211..912477 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KD735_RS04440 (912458) 912458..912865 + 408 WP_000244781.1 protein YgfX Toxin
KD735_RS04445 (912905) 912905..913426 - 522 WP_001055874.1 flavodoxin FldB -
KD735_RS04450 (913538) 913538..914434 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KD735_RS04455 (914459) 914459..915169 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KD735_RS04460 (915175) 915175..916908 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T199909 WP_000244781.1 NZ_CP073624:912458-912865 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199909 NZ_CP097658:355402-355749 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199909 WP_000354046.1 NZ_CP073624:912211-912477 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199909 NZ_CP097658:355749-356048 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References