Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 349676..350307 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag KD735_RS01555 Protein ID WP_001259385.1
Coordinates 349676..349951 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KD735_RS01560 Protein ID WP_000593555.1
Coordinates 349948..350307 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS01540 (344680) 344680..345747 + 1068 WP_000361470.1 HlyD family secretion protein -
KD735_RS01545 (345744) 345744..348479 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
KD735_RS01550 (348479) 348479..349603 + 1125 WP_001488398.1 ABC transporter permease -
KD735_RS01555 (349676) 349676..349951 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
KD735_RS01560 (349948) 349948..350307 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KD735_RS01565 (350427) 350427..350828 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KD735_RS01570 (350834) 350834..351640 - 807 WP_054626010.1 nickel import ATP-binding protein NikE -
KD735_RS01575 (351637) 351637..352401 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KD735_RS01580 (352401) 352401..353234 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KD735_RS01585 (353231) 353231..354175 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 278957..351640 72683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T199905 WP_001259385.1 NZ_CP073624:349676-349951 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T199905 NZ_CP097656:30888-31145 [Klebsiella pneumoniae]
ATGAAAAAGTCAAAGGGTGATGCTCAATATTATCTTGAGAAGGAAGGTGATATATATCACCTTGTCAAAAGAGTTAAAAC
ATTCTCAAAAAAATTGACTCAGGGTAAAACGAAAGCAACAACGAAAACGGTCAGTGACTTTAGTTTTACTAAAAATAATT
TCGAGGATATAGATTTTAATGCAAATGGTTTAAGGGAGAAAGATAAGTCAATAATTGTTCAGATGGTTGAAGAAATCGAG
GGGCTACATGCTGATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT199905 WP_000593555.1 NZ_CP073624:349948-350307 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT199905 NZ_CP097656:31135-31368 [Klebsiella pneumoniae]
ATGCTGATTAATACGCCTGAAAACATCAAAATTTTGCGAAAAAAAATAGGGCTAACGCAGAAAGAATGTGCGGAAATGTT
CGGAATGACCGCGCGATCATGGCGGCGTAAGGAAGAACCACAAACGACAGCCAGCAATACAACACTATCGCCAATAGAAT
TTAACTATCTTTTGTTACTGGCTGGCGAGCATCCTGATTATGTTTTGTGCAAGCGCGAGGCATTGCACCCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References