Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 60987..62610 Replicon plasmid pCE2050_B
Accession NZ_CP073623
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KEW59_RS24600 Protein ID WP_000088046.1
Coordinates 60987..62312 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KEW59_RS24605 Protein ID WP_024136327.1
Coordinates 62317..62610 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS24570 (KEW59_24385) 56971..57828 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KEW59_RS24575 (KEW59_24390) 57844..58152 - 309 WP_001224687.1 hypothetical protein -
KEW59_RS24580 (KEW59_24395) 58701..59126 - 426 WP_001043843.1 hypothetical protein -
KEW59_RS24585 (KEW59_24400) 59380..60195 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KEW59_RS24590 (KEW59_24405) 60208..60618 - 411 WP_000985911.1 hypothetical protein -
KEW59_RS24595 (KEW59_24410) 60720..60926 - 207 WP_000134171.1 hypothetical protein -
KEW59_RS24600 (KEW59_24415) 60987..62312 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KEW59_RS24605 (KEW59_24420) 62317..62610 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KEW59_RS24610 (KEW59_24425) 63214..63606 - 393 WP_000341066.1 hypothetical protein -
KEW59_RS24615 (KEW59_24430) 63979..64320 + 342 WP_001151575.1 hypothetical protein -
KEW59_RS24620 (KEW59_24435) 64412..64603 + 192 WP_000398480.1 hypothetical protein -
KEW59_RS24625 (KEW59_24440) 64653..64934 + 282 WP_000077926.1 hypothetical protein -
KEW59_RS25430 65275..65406 - 132 WP_000374059.1 hypothetical protein -
KEW59_RS24630 (KEW59_24445) 65497..66738 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA2 / dfrA12 / tet(A) - 1..222008 222008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T199903 WP_000088046.1 NZ_CP073623:c62312-60987 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T199903 NZ_CP097655:6634-7050 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT199903 WP_024136327.1 NZ_CP073623:c62610-62317 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT199903 NZ_CP097655:6407-6637 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References