Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57946..58471 Replicon plasmid pCE2050_A
Accession NZ_CP073622
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KEW59_RS23925 Protein ID WP_001159871.1
Coordinates 57946..58251 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KEW59_RS23930 Protein ID WP_000813634.1
Coordinates 58253..58471 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS23895 (53213) 53213..54483 + 1271 Protein_70 translesion error-prone DNA polymerase V subunit UmuC -
KEW59_RS23900 (54488) 54488..54880 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
KEW59_RS23905 (54885) 54885..55856 - 972 WP_001103694.1 plasmid segregation protein ParM -
KEW59_RS23910 (56085) 56085..56729 + 645 WP_000633911.1 AAA family ATPase -
KEW59_RS23915 (56723) 56723..56998 + 276 WP_000239529.1 hypothetical protein -
KEW59_RS23920 (57136) 57136..57945 - 810 WP_000016979.1 site-specific integrase -
KEW59_RS23925 (57946) 57946..58251 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KEW59_RS23930 (58253) 58253..58471 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KEW59_RS23935 (60793) 60793..61434 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..115443 115443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T199902 WP_001159871.1 NZ_CP073622:c58251-57946 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T199902 NZ_CP097655:c5599-5294 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT199902 WP_000813634.1 NZ_CP073622:c58471-58253 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT199902 NZ_CP097655:c5819-5601 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References