Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3284660..3285314 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KEW59_RS15625 Protein ID WP_000244781.1
Coordinates 3284907..3285314 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KEW59_RS15620 Protein ID WP_000354046.1
Coordinates 3284660..3284926 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS15600 (3280748) 3280748..3282181 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
KEW59_RS15605 (3282226) 3282226..3282537 + 312 WP_001525592.1 N(4)-acetylcytidine aminohydrolase -
KEW59_RS15610 (3282701) 3282701..3283360 + 660 WP_000250275.1 hemolysin III family protein -
KEW59_RS15615 (3283437) 3283437..3284417 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
KEW59_RS15620 (3284660) 3284660..3284926 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KEW59_RS15625 (3284907) 3284907..3285314 + 408 WP_000244781.1 protein YgfX Toxin
KEW59_RS15630 (3285354) 3285354..3285875 - 522 WP_001055874.1 flavodoxin FldB -
KEW59_RS15635 (3285987) 3285987..3286883 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KEW59_RS15640 (3286908) 3286908..3287618 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KEW59_RS15645 (3287624) 3287624..3289357 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T199888 WP_000244781.1 NZ_CP073621:3284907-3285314 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199888 NZ_CP097652:35471-35782 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199888 WP_000354046.1 NZ_CP073621:3284660-3284926 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199888 NZ_CP097652:35779-36198 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References