Detailed information of TA system TA199887

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3156218..3157013
• Replicon: chromosome
• Accession: NZ_CP073621
• Organism: Escherichia coli strain CE2050

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A658LKE6
• Locus tag: KEW59_RS14920
• Protein ID: WP_001596171.1
• Coordinates: 3156218..3156592 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KEW59_RS14925
• Protein ID: WP_001596170.1
• Coordinates: 3156639..3157013 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEW59_RS14895 (3152288)	3152288..3153271	-	984	WP_001596176.1	KpsF/GutQ family sugar-phosphate isomerase	-
KEW59_RS14900 (3154082)	3154082..3154252	-	171	Protein_2900	IS110 family transposase	-
KEW59_RS14905 (3154594)	3154594..3155436	-	843	Protein_2901	DUF4942 domain-containing protein	-
KEW59_RS14910 (3155521)	3155521..3155718	-	198	WP_001596173.1	DUF957 domain-containing protein	-
KEW59_RS14915 (3155730)	3155730..3156221	-	492	WP_001596172.1	DUF5983 family protein	-
KEW59_RS14920 (3156218)	3156218..3156592	-	375	WP_001596171.1	TA system toxin CbtA family protein	Toxin
KEW59_RS14925 (3156639)	3156639..3157013	-	375	WP_001596170.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KEW59_RS14930 (3157176)	3157176..3157397	-	222	WP_000692323.1	DUF987 domain-containing protein	-
KEW59_RS14935 (3157460)	3157460..3157936	-	477	WP_001186726.1	RadC family protein	-
KEW59_RS14940 (3157952)	3157952..3158389	-	438	WP_229018344.1	antirestriction protein	-
KEW59_RS14955 (3159790)	3159790..3160272	-	483	Protein_2910	DUF932 domain-containing protein	-
KEW59_RS14960 (3160396)	3160396..3160629	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3154082..3159569	5487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14161.14 Da
• Isoelectric Point: 6.7041

>T199887 WP_001596171.1 NZ_CP073621:c3156592-3156218 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLARLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYELVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T199887 NZ_CP097651:c5322730-5322347 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13813.62 Da
• Isoelectric Point: 6.7387

>AT199887 WP_001596170.1 NZ_CP073621:c3157013-3156639 [Escherichia coli]
VSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT199887 NZ_CP097651:c5322972-5322730 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A658LKE6