Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1508588..1509164 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag KEW59_RS07070 Protein ID WP_001131965.1
Coordinates 1508877..1509164 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag KEW59_RS07065 Protein ID WP_019842999.1
Coordinates 1508588..1508890 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS07045 (1504282) 1504282..1504794 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
KEW59_RS07050 (1505184) 1505184..1507334 + 2151 WP_001439331.1 pyruvate/proton symporter BtsT -
KEW59_RS07055 (1507384) 1507384..1507587 + 204 WP_000467859.1 YbdD/YjiX family protein -
KEW59_RS07060 (1507598) 1507598..1508554 + 957 WP_001295525.1 GTPase -
KEW59_RS07065 (1508588) 1508588..1508890 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
KEW59_RS07070 (1508877) 1508877..1509164 - 288 WP_001131965.1 BrnT family toxin Toxin
KEW59_RS07075 (1509361) 1509361..1510527 + 1167 WP_000800825.1 restriction endonuclease -
KEW59_RS07080 (1510591) 1510591..1512210 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -
KEW59_RS07085 (1512200) 1512200..1513624 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T199881 WP_001131965.1 NZ_CP073621:c1509164-1508877 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T199881 NZ_CP097651:799818-800228 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT199881 WP_019842999.1 NZ_CP073621:c1508890-1508588 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT199881 NZ_CP097651:799571-799837 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References