Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 932103..932721 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KEW59_RS04370 Protein ID WP_001291435.1
Coordinates 932503..932721 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KEW59_RS04365 Protein ID WP_000344800.1
Coordinates 932103..932477 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS04355 (927192) 927192..928385 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KEW59_RS04360 (928408) 928408..931557 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KEW59_RS04365 (932103) 932103..932477 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KEW59_RS04370 (932503) 932503..932721 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KEW59_RS04375 (932893) 932893..933444 + 552 WP_000102564.1 maltose O-acetyltransferase -
KEW59_RS04380 (933560) 933560..934030 + 471 WP_000136192.1 YlaC family protein -
KEW59_RS04385 (934194) 934194..935744 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KEW59_RS04390 (935786) 935786..936139 - 354 WP_001550741.1 DUF1428 family protein -
KEW59_RS04400 (936518) 936518..936829 + 312 WP_000409911.1 MGMT family protein -
KEW59_RS04405 (936860) 936860..937432 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199878 WP_001291435.1 NZ_CP073621:932503-932721 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T199878 NZ_CP097649:c2977297-2975987 [Brevundimonas albigilva]
ATGCCTGTGCTGTTCCATCGCGACAGCCCGGTCGCCAAGGTGACGCCGTCGCCGGCGGGGCTGAACCTGATCTATGACGA
GACCTGGCGCGCCTCGCCGGAAGCCTTTCCGGTCTCGCTGTCCATGCCGATGGCGGAGGCCGCATGGCCGCCCGAGGTGG
TCGTCCCTTGGGTGATGAACCTGCTGCCCGAAGGCGAGCCCATGCGCGCCATGCAGCGCGCGCTCGGCGTCGCTCAAGAG
GATGTGCTCGGCCTCCTCTCGGCCGCTGGCGGCGACGTCGCAGGCGCCCTGCGCGTCGGCGGCCCCCGCGCGGAGCAGGG
CGACTACCGCCCCCTACCGACGCCGGATGATCTGGAACGGATCATCGGCGAGTTGCCTCGCAAGCCTTTCCTGGCAGGGG
ACAAGGGCGTGACCATGAGTCTGGCCGGCGCTCAGGACAAGTTGCCGGTGGCGCTGCTGAATGGTCAGATCGTCATCCCG
CTGGATGGTGCGCCTTCGACCCACATTCTGAAGCCCGACAACCCCCGCCTGCCCGGCAGCGTCCAGAACGAAGCGTTGTG
CATGCTGCTCGCCGCGCGGTGCCGACTGCCGACGGCTTCAGTCACGACCGGTCGGGCTGGCGACCGTTCCTATCTGCTGG
TCGATCGCTATGACCGCGCGGGCCATGCGCCTGCCGCCACGCGGCTGCACCAGGAGGACTTCTGTCAGGCGCTCCGCTTG
CCGCCGGGCGCCAAATACGAGCGCAACCAAACCGGCGTGCGTGGCCCCTCGTTGGCCGACATGTTCGCTCTCGTGCGTCG
GCACATGACGGCCGTCGACATCACCCGCCTGCTGGACGCGGTGATCTTCAACATCGCCATTGGCAACGTCGATTCCCACG
CCAAAAACTACTCGATCCTGCTGACCGCAGACGGCGCGGCCCTGGCTCCGCTCTACGACTTGATGAGCGGCCTGCCGTGG
CCGGGCATCACCCAGAACCACGCCCAGGCCGTCGGCGGTCAGGTCCGCGGCCGCCATATCCAGGCCCGGCACTGGCGCCG
GATGGCTGAGGCCTGCGGCCTGGCGCCGGCCGCCACTGTGCGGCGGGTCATCGCCCTGACCGACCGCATCCAGCGCGAGA
TCGGCGCGGCGGCCGAGGCGGTCGCCGCGATGCCGGCCGGCGGCGGCGCCTTCCTGGACGGTCTCATGCGTAACATCACC
GAGCGCGCCGCCCTTGTCGGCCGCAACGCTGCTCGTGATGACGGTGGAGATGACGCTGACGGCGAGGGACCGGCCGTCCG
CGGCGAGGCCTTCCCGTCTTCGTCGAACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT199878 WP_000344800.1 NZ_CP073621:932103-932477 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT199878 NZ_CP097649:c2977509-2977297 [Brevundimonas albigilva]
ATGATCGTCACTGCGGAAGATTTCGGCCGGCAGGTCGCCGAGGCGCGCCGCGCTCTTGGCCTGACCCAGCGCGACCTCGC
CCTGGCGATCGGAACCGGCGAGCGGTTTATTGTCGAGCTGGAGGCGGGCAAGGCCACCGCCCAACTCGGCAAGGCGCTTG
CGGCCGCCAAGGCGGTCGGCGTTCGCTTGGAGAACACGGCGGGGCGTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References