Detailed information of TA system TA199877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 529508..530213
• Replicon: chromosome
• Accession: NZ_CP073621
• Organism: Escherichia coli strain CE2050

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: KEW59_RS02600
• Protein ID: WP_000539521.1
• Coordinates: 529508..529894 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KEW59_RS02605
• Protein ID: WP_001280945.1
• Coordinates: 529884..530213 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEW59_RS02580 (525512)	525512..526138	+	627	WP_001595548.1	glutathione S-transferase GstB	-
KEW59_RS02585 (526135)	526135..527250	-	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
KEW59_RS02590 (527361)	527361..527744	-	384	WP_000497137.1	biofilm formation regulator BssR	-
KEW59_RS02595 (527957)	527957..529282	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KEW59_RS02600 (529508)	529508..529894	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEW59_RS02605 (529884)	529884..530213	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KEW59_RS02610 (530283)	530283..531611	-	1329	WP_000086872.1	GGDEF domain-containing protein	-
KEW59_RS02615 (531619)	531619..533967	-	2349	WP_001595546.1	EAL domain-containing protein	-
KEW59_RS02620 (534145)	534145..535056	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T199877 WP_000539521.1 NZ_CP073621:529508-529894 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T199877 NZ_CP097649:2862237-2862653 [Brevundimonas albigilva]
GTGCTGATCGCCCTGGTCGATCCGCGTCACGTCTTTCACGATCCGGCGAATGCCTGGTTTCATCGCGAGGCCGTCGCTTC
CTGGGCGACCTGTCCGATCACCGAGAACGGTTTGGTCCGGATCGTCGGCAGCAACCGCTATCGCAATCCGGTCGGCCCTC
CGTCCGAAGTGCTTCGAATCCTCGACCTTCTGCGCGACATGCGAGGACACGTCTTCTGGCCCGATACGGTCAGTCTGGCG
AACGGCCGGCTGTTCAACCCGTCGGCCCTGACCGCACCTGAACAAATCACGGATGCTTATCTGCTGGCCTTGGCGGTCTC
GAACGGCGGCCTTCTGGCGACCTTTGACCGCCGCCTTTCGCCGGACGCCGTCAAGGGCGGGCGAGCGGCCCTCCATCTGA
TCGCCGACACCCGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT199877 WP_001280945.1 NZ_CP073621:529884-530213 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT199877 NZ_CP097649:2861989-2862216 [Brevundimonas albigilva]
ATGCGCACCACCCTGGCCATCGACGACGACGTGCTGAACGCCGCGAGGGCCATCGCGGCGCATCAAGACCGCACAGTCGG
GGAAGTCCTGTCCGAACTTGCGCGACAGACGTTGCAAAAACGACCGACGACGACCCGTTTCCGCAATGGCGTCCCTCTTC
TGCCCGATCGGCCGGGACCGATGGTCACATTGGAAGACGTCAACGCGCTGCGCGACGAACTGCCTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6