Detailed information of TA system TA199876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 274800..275584
• Replicon: chromosome
• Accession: NZ_CP073621
• Organism: Escherichia coli strain CE2050

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: KEW59_RS01395
• Protein ID: WP_001595626.1
• Coordinates: 275090..275584 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: KEW59_RS01390
• Protein ID: WP_001110447.1
• Coordinates: 274800..275093 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEW59_RS01380 (269950)	269950..270909	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
KEW59_RS01385 (271482)	271482..274667	+	3186	WP_001595627.1	ribonuclease E	-
KEW59_RS01390 (274800)	274800..275093	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
KEW59_RS01395 (275090)	275090..275584	+	495	WP_001595626.1	GNAT family N-acetyltransferase	Toxin
KEW59_RS01400 (275679)	275679..276632	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
KEW59_RS01405 (276644)	276644..278287	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
KEW59_RS01410 (278353)	278353..279294	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
KEW59_RS01415 (279294)	279294..280391	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18065.97 Da
• Isoelectric Point: 8.2617

>T199876 WP_001595626.1 NZ_CP073621:275090-275584 [Escherichia coli]
MIQAPEPLIARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T199876 NZ_CP097649:2688226-2688471 [Brevundimonas albigilva]
GTGAAAACCATCGTCCTGACCAACAGTGCGGCCAGACAGTTCGACGCCTTGCCCGCGTCGGCCCGGGCGACGATCTCTGC
CGCCCTGGACGCCTATGCGATGCACGGGCGCGGCGACGTCAAGGCGCTCAACGGACGGCTCGGCTTTCGGCTCAGGGTCG
GGCGCTATCGGGTGATCTTCACCGAGGACATGACCACCATCCTGGCCATCCATATCGGACCACGCCAGACCGGAACCTAC
AGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT199876 WP_001110447.1 NZ_CP073621:274800-275093 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT199876 NZ_CP097649:2688468-2688860 [Brevundimonas albigilva]
ATGAATCGTCCCCTTCACGCGCAACACATCACCACGCCCGCCGGCGAGGAGCTGGTCATCCTGCCCCGCGCCGAATACGA
GGCGCTGACGGCCGGTCTCGACCAGGACGAGGACGAGGCCGATATCGCCGCCTTCGACGCGGCCATGGCCGATCTCGAAG
CCGGCGTGGACGAACGCCTGCCGGTCGAGGTCTCGACGCTGATCCTGAAGGGCGCTTCGCGGCTGACGGCCTGGCGCAAA
CATCGCGGCTGGACCCAGCAGGCGCTGGCCGAGGCCGCGACCATCGGCCAGGGCTACCTGTCGGAGATCGAGCGGGGGCA
CAAGACCGGCGGCCCCGAAACGCTGCGACGCCTGGCCGAGGTGCTGGACGTGCCGGCGGACCACATCGGATGA

Structures

Antitoxin

• AlphaFold DB: L4JCW6