Detailed information of TA system TA199871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 30212..30737
• Replicon: plasmid pMFDS1006657
• Accession: NZ_CP073590
• Organism: Escherichia coli strain MFDS1006657

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: KD154_RS24425
• Protein ID: WP_001159871.1
• Coordinates: 30432..30737 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: KD154_RS24420
• Protein ID: WP_000813639.1
• Coordinates: 30212..30430 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD154_RS24385 (26344)	26344..26496	+	153	Protein_22	IS481 family transposase	-
KD154_RS24390 (26773)	26773..27504	+	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
KD154_RS24395 (27614)	27614..27934	+	321	WP_001365556.1	colicin-like pore-forming protein	-
KD154_RS24400 (27941)	27941..28150	-	210	WP_225303885.1	colicin E1 immunity protein	-
KD154_RS24405 (28404)	28404..28751	+	348	WP_000142428.1	DUF6404 family protein	-
KD154_RS24410 (28769)	28769..29359	-	591	WP_000194564.1	hypothetical protein	-
KD154_RS24415 (29356)	29356..29616	-	261	WP_000343084.1	hypothetical protein	-
KD154_RS24420 (30212)	30212..30430	+	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KD154_RS24425 (30432)	30432..30737	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
KD154_RS24430 (30738)	30738..31547	+	810	WP_000016958.1	site-specific integrase	-
KD154_RS24435 (31685)	31685..31960	-	276	WP_000239527.1	hypothetical protein	-
KD154_RS24440 (31954)	31954..32598	-	645	WP_000633912.1	division plane positioning ATPase MipZ	-
KD154_RS24445 (32827)	32827..33798	+	972	WP_001103695.1	plasmid segregation protein ParM	-
KD154_RS24450 (33803)	33803..34195	+	393	WP_000340836.1	plasmid partitioning/stability family protein	-
KD154_RS24455 (34200)	34200..35470	-	1271	Protein_36	translesion error-prone DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..122855	122855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T199871 WP_001159871.1 NZ_CP073590:30432-30737 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T199871 NZ_CP097647:c38193-37909 [Klebsiella quasipneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT199871 WP_000813639.1 NZ_CP073590:30212-30430 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT199871 NZ_CP097647:c38431-38183 [Klebsiella quasipneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5