Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4537334..4537952 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KD154_RS21845 Protein ID WP_001291435.1
Coordinates 4537334..4537552 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KD154_RS21850 Protein ID WP_000344800.1
Coordinates 4537578..4537952 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS21810 (4532624) 4532624..4533196 + 573 WP_000779826.1 YbaY family lipoprotein -
KD154_RS21815 (4533227) 4533227..4533538 - 312 WP_000409911.1 MGMT family protein -
KD154_RS21825 (4533917) 4533917..4534270 + 354 WP_000878141.1 DUF1428 family protein -
KD154_RS21830 (4534312) 4534312..4535862 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KD154_RS21835 (4536026) 4536026..4536496 - 471 WP_000136192.1 YlaC family protein -
KD154_RS21840 (4536612) 4536612..4537162 - 551 Protein_4298 maltose O-acetyltransferase -
KD154_RS21845 (4537334) 4537334..4537552 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KD154_RS21850 (4537578) 4537578..4537952 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KD154_RS21855 (4538498) 4538498..4541647 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KD154_RS21860 (4541670) 4541670..4542863 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199870 WP_001291435.1 NZ_CP073589:c4537552-4537334 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T199870 NZ_CP097647:24438-24908 [Klebsiella quasipneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGAATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAATTGATCCAAAGCGCAAATATGGAT
GAATTACCGGCCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCTCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGATTTTT
ACGGGATCGTGCAGATGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT199870 WP_000344800.1 NZ_CP073589:c4537952-4537578 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT199870 NZ_CP097647:23998-24441 [Klebsiella quasipneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTGCTGGGTCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AAAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGACTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGATAAGGAAGCCGCACTGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATAGCATCCGAGACGGGAGCATATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References