Detailed information of TA system TA199869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4503039..4503876
• Replicon: chromosome
• Accession: NZ_CP073589
• Organism: Escherichia coli strain MFDS1006657

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KD154_RS21675
• Protein ID: WP_000227784.1
• Coordinates: 4503039..4503581 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KD154_RS21680
• Protein ID: WP_001297137.1
• Coordinates: 4503565..4503876 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD154_RS21655 (4498578)	4498578..4499489	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
KD154_RS21660 (4499657)	4499657..4500148	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
KD154_RS21665 (4500276)	4500276..4501640	-	1365	WP_001000978.1	MFS transporter	-
KD154_RS21670 (4502048)	4502048..4502983	+	936	WP_001387350.1	tetratricopeptide repeat protein	-
KD154_RS21675 (4503039)	4503039..4503581	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KD154_RS21680 (4503565)	4503565..4503876	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KD154_RS21685 (4504061)	4504061..4504951	-	891	WP_000971336.1	heme o synthase	-
KD154_RS21690 (4504963)	4504963..4505292	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KD154_RS21695 (4505292)	4505292..4505906	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KD154_RS21700 (4505896)	4505896..4507887	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KD154_RS21705 (4507909)	4507909..4508856	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T199869 WP_000227784.1 NZ_CP073589:c4503581-4503039 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T199869 NZ_CP097646:c4604329-4604048 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT199869 WP_001297137.1 NZ_CP073589:c4503876-4503565 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT199869 NZ_CP097646:c4604561-4604319 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3