Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2887607..2888193 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KD154_RS14160 Protein ID WP_042014162.1
Coordinates 2887963..2888193 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KD154_RS14155 Protein ID WP_000593555.1
Coordinates 2887607..2887966 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS14130 (2883739) 2883739..2884683 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
KD154_RS14135 (2884680) 2884680..2885513 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KD154_RS14140 (2885513) 2885513..2886277 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KD154_RS14145 (2886274) 2886274..2887080 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KD154_RS14150 (2887086) 2887086..2887487 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KD154_RS14155 (2887607) 2887607..2887966 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KD154_RS14160 (2887963) 2887963..2888193 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KD154_RS14165 (2888297) 2888297..2889421 - 1125 WP_001314210.1 ABC transporter permease -
KD154_RS14170 (2889421) 2889421..2892156 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T199863 WP_042014162.1 NZ_CP073589:c2888193-2887963 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T199863 NZ_CP097646:316410-316778 [Klebsiella quasipneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
TGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT199863 WP_000593555.1 NZ_CP073589:c2887966-2887607 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT199863 NZ_CP097646:316192-316413 [Klebsiella quasipneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATAGATG
ATGAGTTCGCGGCAATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References