Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2302956..2303610 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KD154_RS11220 Protein ID WP_000244772.1
Coordinates 2302956..2303363 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KD154_RS11225 Protein ID WP_000354046.1
Coordinates 2303344..2303610 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS11200 (2298913) 2298913..2300646 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
KD154_RS11205 (2300652) 2300652..2301362 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KD154_RS11210 (2301387) 2301387..2302283 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KD154_RS11215 (2302395) 2302395..2302916 + 522 WP_001055874.1 flavodoxin FldB -
KD154_RS11220 (2302956) 2302956..2303363 - 408 WP_000244772.1 protein YgfX Toxin
KD154_RS11225 (2303344) 2303344..2303610 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KD154_RS11230 (2303853) 2303853..2304833 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KD154_RS11235 (2304910) 2304910..2305569 - 660 WP_000250274.1 hemolysin III family protein -
KD154_RS11240 (2305733) 2305733..2306044 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KD154_RS11245 (2306089) 2306089..2307522 + 1434 WP_040078854.1 6-phospho-beta-glucosidase BglA -
KD154_RS11250 (2307579) 2307579..2308322 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T199860 WP_000244772.1 NZ_CP073589:c2303363-2302956 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199860 NZ_CP097636:c715233-715057 [Aquincola tertiaricarbonis]
GTGCTGCCGCAGTTGCGGCGCAAGTCGCCCGACCCGGTGAGGCGGCTGGCGCGCCCCTTGCCCGCCGTTGACAGACTGCT
GACCGCCACCGCCTTGCATCACGGCCTGATCCTCGTCACGCGCAACGTGCGTGACGTGTCAGACCTCGGCGTACAGGTAC
TGAACCCCTGGGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199860 WP_000354046.1 NZ_CP073589:c2303610-2303344 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199860 NZ_CP097636:c715508-715254 [Aquincola tertiaricarbonis]
ATGCAGACGTGGCAGATGCAGGACGCCAAGGCGCGCCTGTCGGAAGTGGTCAAGCGCGCGGAACAGGATGGCCCGCAGGA
CATCACGGTGCACGGCAAGTCGGTGGCGGTGGTGCTCTCCCGGGCGGCGTTCGAGCGCATGTCGGGCCAGCAGGCTTCAC
TGCTGGCGTTCATGCAGGCGTCGCCTTTGGCAGGGCTGGATGACGAGCTGGTGATCGAGCGCGACGCGAGTCTGACGCGC
GACGTGTCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References