Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2132110..2132693 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KD154_RS10465 Protein ID WP_000254745.1
Coordinates 2132110..2132445 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KD154_RS10470 Protein ID WP_000581937.1
Coordinates 2132445..2132693 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS10450 (2127997) 2127997..2129295 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KD154_RS10455 (2129383) 2129383..2131020 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KD154_RS10460 (2131248) 2131248..2132039 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KD154_RS10465 (2132110) 2132110..2132445 - 336 WP_000254745.1 endoribonuclease MazF Toxin
KD154_RS10470 (2132445) 2132445..2132693 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KD154_RS10475 (2132771) 2132771..2135005 - 2235 WP_000226815.1 GTP pyrophosphokinase -
KD154_RS10480 (2135053) 2135053..2136354 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T199859 WP_000254745.1 NZ_CP073589:c2132445-2132110 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T199859 NZ_CP097635:c2106447-2105092 [Aquincola tertiaricarbonis]
ATGGGCCGTCTGTCGCACCACCGCACGCTGGGGCTCTGGATGAACGGGGAGCGTGTCGGCCGCTGGACCCTGGCGCCCAG
TGCCCCCGACCTGCTGCAGTACGACCCGGCCTGGCTGCAGTCCGAAGCCGGGCGCCCACTGTCGCTGTCCCTGCCCTTTC
TGCCCGGCAACGCACCCCACCGCGGCCCAGCGGTGCGCGCGTACTTCGAGAACCTGCTGCCCGACAGCCGCGACATCCGC
GAGCGCCTGGCGCGCCGCTTCGGCGTCGGCTCCACCTCGGCTTTCGACCTGCTGGCCGAGATCGGCCGCGACTGCGTGGG
TGCGCTGGAGATCCTGCCCGACGACCAGAGCTCCAACGGCGTGGCACCCGTGAAAGCCGAACCGCTGACCGAAGCCCAGG
TGGCCCAGCTGCTGCGCAGCGCCACCACGCCGCAGGCCCTGGGCCGCGGCACCGACGACGAGGACTTCCGCATCTCCATC
GCCGGCGCCCAGGAAAAAACCGCGCTGCTGTGGCACGGCGGCCAGTGGTGCCTGCCCCGCGGCAGCACGCCCACCAGCCA
CATCCTGAAGCTGCCGCTGGGGCTGGTGGGCGGCATGCGGCTCGATCTGCGCGACTCGGTGGAGAACGAGTGGCTGTGCG
CGCTCATCCTGAAGGCCTTCGGCCTGCCGGTGGCCACCTGCCACCCGCTGCAGTTCGAGGACGTGAAGGTGCTGGGCATC
GAGCGCTTTGATCGCAGTTGGAGGTCGCCACCCCGGGGCGCGCAGCGCCTGCTGCGCCTGCCCCAGGAAGACCTGTGCCA
GGCCACTGGCGTGCCGCCGGAAGCCAAATACGAGGCCGACGGCGGACCGGGGATGGACCGCATCCTGGGCGTGCTGGATG
GCTCGACCGAACGGGCGCAGGACAGGCGCCACTTCTTCCAGGCGCAGCTGTTGTTCTGGATGCTGTGCGCCACCGATGGC
CATGCCAAGAACTTCAGCCTCTTCCTGCGGCCTGGTGGCCGCTACCAGCTGACGCCGCTGTACGACGTGCTGTCGGCCTA
CCCGGTGCTGGGCGATGGGCCCGCCCGCATCTCAGCCCACCGGGCACGCATGGCCATGGCCGTGCGCTCGAAGAACGCCC
ACTGGCGCATGCGCGACATCCACCGCCGGCACTGGGTGGCGCTAGGCATGCGCCACGGCATCACCACCGAAGACGGGCGG
GGCGTGGAGGCGCTGATCGACGAGCTGGTGGCCCGCACCCCGGCCGTCATCGCCGCGGTGCAGGGGCAGTTGCCGGCGTG
GTTCCCGCAGCGGGTGGCCGCCAGCATCTTCAGCGGGCTGGCGGGGGCTGCAAGACGCCTGGGCGCTGCAGCTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT199859 WP_000581937.1 NZ_CP073589:c2132693-2132445 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT199859 NZ_CP097635:c2106718-2106452 [Aquincola tertiaricarbonis]
ATGCAGAAGTTTCACCTTCGCACGGCCGACCAGCTGCCCGCGCTGCTGCAGGCCTTTCGCAAGCAGGCAGGCCTCACGCA
GCGTGAGGTGGCGCTGCGCCTGGGCGTCACGCAGCAAACGTACTCGGCGCTGGAACGCAATGCCGACACCGTGGGCGCCG
CCCGGCTGCTGAAGCTGCTGCACATCCTGGGCGTGGAATGGGTGCTGACCCAGCCCGACCCAGACGCCGAGGCACCACTG
TCGGTGGCCGAGACGCCTCCCTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References