Detailed information of TA system TA199839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 143590..144115
• Replicon: plasmid p00003
• Accession: NZ_CP073380
• Organism: Klebsiella sp. P1927

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: KB892_RS30910
• Protein ID: WP_013023785.1
• Coordinates: 143590..143895 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: KB892_RS30915
• Protein ID: WP_001568025.1
• Coordinates: 143897..144115 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB892_RS30880 (139277)	139277..139903	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
KB892_RS31400 (139900)	139900..140202	+	303	WP_004197636.1	hypothetical protein	-
KB892_RS30890 (140666)	140666..141460	-	795	WP_004197635.1	site-specific integrase	-
KB892_RS30895 (141658)	141658..142674	-	1017	WP_017899884.1	hypothetical protein	-
KB892_RS30900 (142685)	142685..142999	-	315	WP_053389906.1	hypothetical protein	-
KB892_RS30905 (143026)	143026..143421	-	396	WP_017899885.1	hypothetical protein	-
KB892_RS30910 (143590)	143590..143895	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
KB892_RS30915 (143897)	143897..144115	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KB892_RS30920 (144285)	144285..144746	-	462	WP_160880186.1	hypothetical protein	-
KB892_RS30925 (144703)	144703..144933	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
KB892_RS30930 (144930)	144930..145346	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
KB892_RS30935 (145420)	145420..146982	+	1563	WP_004206609.1	AAA family ATPase	-
KB892_RS30940 (146967)	146967..147989	+	1023	WP_000361404.1	helicase UvrD	-
KB892_RS30945 (148245)	148245..148942	+	698	WP_223174630.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / catA2 / blaKPC-2	-	1..162093	162093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T199839 WP_013023785.1 NZ_CP073380:c143895-143590 [Klebsiella sp. P1927]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T199839 NZ_CP097626:352792-353139 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT199839 WP_001568025.1 NZ_CP073380:c144115-143897 [Klebsiella sp. P1927]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT199839 NZ_CP097626:353139-353438 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8