Detailed information of TA system TA199828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 45193..45715
• Replicon: plasmid p00001
• Accession: NZ_CP073378
• Organism: Klebsiella sp. P1927

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: KB892_RS27485
• Protein ID: WP_004181778.1
• Coordinates: 45431..45715 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: KB892_RS27480
• Protein ID: WP_004181777.1
• Coordinates: 45193..45441 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB892_RS27460 (KB892_27460)	40402..41223	-	822	WP_004181772.1	hypothetical protein	-
KB892_RS27465 (KB892_27465)	41285..41638	-	354	WP_004181774.1	hypothetical protein	-
KB892_RS27470 (KB892_27470)	41783..42769	-	987	WP_101992392.1	hypothetical protein	-
KB892_RS27475 (KB892_27475)	43103..44902	-	1800	WP_004181776.1	ATP-dependent helicase	-
KB892_RS27480 (KB892_27480)	45193..45441	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KB892_RS27485 (KB892_27485)	45431..45715	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KB892_RS27490 (KB892_27490)	45732..45833	-	102	Protein_52	IS200/IS605 family transposase	-
KB892_RS27495 (KB892_27495)	45869..47095	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
KB892_RS27500 (KB892_27500)	47366..47590	-	225	Protein_54	transposase	-
KB892_RS27505 (KB892_27505)	47669..48097	-	429	WP_077257669.1	IS200/IS605 family transposase	-
KB892_RS27510 (KB892_27510)	48133..49320	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
KB892_RS31245	49365..49736	-	372	WP_040209644.1	hypothetical protein	-
KB892_RS31250	49733..50077	-	345	WP_228306996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / aac(3)-IId / blaSHV-12 / qnrB4 / blaDHA-1 / sul1 / armA / msr(E) / mph(E)	rmpA / iutA / iucD / iucC / iucB / iucA	1..299258	299258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T199828 WP_004181778.1 NZ_CP073378:45431-45715 [Klebsiella sp. P1927]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T199828 NZ_CP097608:2124450-2124740 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTGAAGCCATTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCCGCATATTTAAG
TGAGGTTTTAGAAACAGGTGATACTAATGAATTTATTTCCGCGCTAGGTGATGTCGCAAGAGCAAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACAATA
ATGAAAATTACGCAAGCATTAGGCATTAAACTTGTCCCAACGCACATCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT199828 WP_004181777.1 NZ_CP073378:45193-45441 [Klebsiella sp. P1927]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT199828 NZ_CP097608:2124139-2124453 [Pasteurella multocida]
ATGTTTGAGATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAAGATTTGTCGGCTAAAGCAGCTATTCT
TGCTCGTATTAATAGAGCTAAAAATGGTAATTTTGGCGATCACAAATCAGTTGGTGATGGCTTGTATGAAATGAGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTACACACAATACAAAGATGTAACCTATTTGCTAATTTGCGGAGGTGATAAATCT
ACACAGAAAGCAGACATCGCTAAAGCAAAAGTGTTATGGGAAGAGATTAAGCAGAAAGAGGGGGTAAATGTATGA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8