Detailed information of TA system TA199826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 5477923..5478509
• Replicon: chromosome
• Accession: NZ_CP073377
• Organism: Klebsiella sp. P1927

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KB892_RS27145
• Protein ID: WP_002920800.1
• Coordinates: 5478141..5478509 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: KB892_RS27140
• Protein ID: WP_002920802.1
• Coordinates: 5477923..5478144 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB892_RS27120 (KB892_27120)	5474080..5475006	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KB892_RS27125 (KB892_27125)	5475003..5476280	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
KB892_RS27130 (KB892_27130)	5476277..5477044	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KB892_RS27135 (KB892_27135)	5477046..5477759	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KB892_RS27140 (KB892_27140)	5477923..5478144	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KB892_RS27145 (KB892_27145)	5478141..5478509	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KB892_RS27150 (KB892_27150)	5478782..5480098	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KB892_RS27155 (KB892_27155)	5480205..5481092	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KB892_RS27160 (KB892_27160)	5481089..5481934	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KB892_RS27165 (KB892_27165)	5481936..5483006	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5475003..5483743	8740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T199826 WP_002920800.1 NZ_CP073377:5478141-5478509 [Klebsiella sp. P1927]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T199826 NZ_CP097606:449708-450001 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAAGTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTATGCATTCCTTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT199826 WP_002920802.1 NZ_CP073377:5477923-5478144 [Klebsiella sp. P1927]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT199826 NZ_CP097606:449427-449711 [Pasteurella multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTGCTCGTATTAACAAAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4