Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5067483..5068108 Replicon chromosome
Accession NZ_CP073377
Organism Klebsiella sp. P1927

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KB892_RS25230 Protein ID WP_002882817.1
Coordinates 5067483..5067866 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KB892_RS25235 Protein ID WP_004150355.1
Coordinates 5067866..5068108 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB892_RS25215 (KB892_25215) 5064849..5065751 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KB892_RS25220 (KB892_25220) 5065748..5066383 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KB892_RS25225 (KB892_25225) 5066380..5067309 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KB892_RS25230 (KB892_25230) 5067483..5067866 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KB892_RS25235 (KB892_25235) 5067866..5068108 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KB892_RS25240 (KB892_25240) 5068313..5069230 + 918 WP_002882812.1 alpha/beta hydrolase -
KB892_RS25245 (KB892_25245) 5069244..5070185 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KB892_RS25250 (KB892_25250) 5070230..5070667 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KB892_RS25255 (KB892_25255) 5070664..5071524 - 861 WP_002882807.1 virulence factor BrkB family protein -
KB892_RS25260 (KB892_25260) 5071518..5072117 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199825 WP_002882817.1 NZ_CP073377:c5067866-5067483 [Klebsiella sp. P1927]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199825 NZ_CP097606:449427-449711 [Pasteurella multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTGCTCGTATTAACAAAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199825 WP_004150355.1 NZ_CP073377:c5068108-5067866 [Klebsiella sp. P1927]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199825 NZ_CP097606:449708-450001 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAAGTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTATGCATTCCTTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References