Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4494826..4495636 Replicon chromosome
Accession NZ_CP073377
Organism Klebsiella sp. P1927

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KB892_RS22500 Protein ID WP_002887280.1
Coordinates 4494826..4495359 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KB892_RS22505 Protein ID WP_002887278.1
Coordinates 4495370..4495636 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB892_RS22495 (KB892_22495) 4493657..4494778 + 1122 WP_002887282.1 cupin domain-containing protein -
KB892_RS22500 (KB892_22500) 4494826..4495359 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KB892_RS22505 (KB892_22505) 4495370..4495636 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KB892_RS22510 (KB892_22510) 4495739..4497172 - 1434 WP_206659713.1 Cu(+)/Ag(+) sensor histidine kinase -
KB892_RS22515 (KB892_22515) 4497162..4497845 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KB892_RS22520 (KB892_22520) 4498017..4499402 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KB892_RS22525 (KB892_22525) 4499420..4499764 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T199822 WP_002887280.1 NZ_CP073377:c4495359-4494826 [Klebsiella sp. P1927]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T199822 NZ_CP097604:594247-594561 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAGGCGGCTATTCT
TGCTCGTATTAGTCGCGCTAAAAGTGGTAATTTTGGCGATCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGGGCTGGTTATAGGGTTTATTATGCACAATACAAAGATATAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAAATATTGCTAAAGCAAAAGCGTTATGGGAAGAAATTAAGCAGAAAGAGGGGGTAAATGTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT199822 WP_002887278.1 NZ_CP073377:c4495636-4495370 [Klebsiella sp. P1927]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT199822 NZ_CP097604:594558-594848 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCAGCATATTTAAG
TGAAGTTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTTGCAAGAGCGAGAGGAATGACGGAAT
TAGCAGAAAAAACGGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCACACGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References