Detailed information of TA system TA199810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 395554..396329
• Replicon: chromosome
• Accession: NZ_CP073377
• Organism: Klebsiella sp. P1927

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A1Q4Q548
• Locus tag: KB892_RS02100
• Protein ID: WP_004150910.1
• Coordinates: 395844..396329 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: KB892_RS02095
• Protein ID: WP_004150912.1
• Coordinates: 395554..395847 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB892_RS02075 (KB892_02075)	390762..391364	-	603	WP_062954968.1	short chain dehydrogenase	-
KB892_RS02080 (KB892_02080)	391462..392373	+	912	WP_004188550.1	LysR family transcriptional regulator	-
KB892_RS02085 (KB892_02085)	392374..393522	-	1149	WP_004150915.1	PLP-dependent aspartate aminotransferase family protein	-
KB892_RS02090 (KB892_02090)	393533..394909	-	1377	WP_004217775.1	cystathionine beta-synthase	-
KB892_RS02095 (KB892_02095)	395554..395847	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
KB892_RS02100 (KB892_02100)	395844..396329	+	486	WP_004150910.1	GNAT family N-acetyltransferase	Toxin
KB892_RS02105 (KB892_02105)	397033..397626	+	594	WP_004188553.1	hypothetical protein	-
KB892_RS02110 (KB892_02110)	397723..397939	+	217	Protein_413	transposase	-
KB892_RS02115 (KB892_02115)	398545..399417	+	873	WP_004188557.1	ParA family protein	-
KB892_RS02120 (KB892_02120)	399417..399800	+	384	WP_004150906.1	hypothetical protein	-
KB892_RS02125 (KB892_02125)	399793..401160	+	1368	WP_004150905.1	SPI-7-type island replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	397723..397875	152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17551.59 Da
• Isoelectric Point: 8.8818

>T199810 WP_004150910.1 NZ_CP073377:395844-396329 [Klebsiella sp. P1927]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T199810 NZ_CP097589:1422463-1423209 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAAGCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT199810 WP_004150912.1 NZ_CP073377:395554-395847 [Klebsiella sp. P1927]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT199810 NZ_CP097589:1423210-1423380 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A1Q4Q548

Antitoxin

• AlphaFold DB: A0A0H3GVL4