Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 70619..71262 Replicon plasmid p00002
Accession NZ_CP073374
Organism Klebsiella sp. P1954

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KB890_RS26450 Protein ID WP_001044770.1
Coordinates 70846..71262 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KB890_RS26445 Protein ID WP_001261282.1
Coordinates 70619..70849 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB890_RS27635 (65840) 65840..66142 + 303 WP_004197636.1 hypothetical protein -
KB890_RS26410 (66582) 66582..67376 - 795 WP_004197635.1 site-specific integrase -
KB890_RS26415 (67574) 67574..68590 - 1017 WP_017899884.1 hypothetical protein -
KB890_RS26420 (68601) 68601..68915 - 315 WP_053389906.1 hypothetical protein -
KB890_RS26425 (68942) 68942..69337 - 396 WP_017899885.1 hypothetical protein -
KB890_RS26430 (69506) 69506..69811 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
KB890_RS26435 (69813) 69813..70031 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
KB890_RS26440 (70201) 70201..70662 - 462 WP_160880186.1 hypothetical protein -
KB890_RS26445 (70619) 70619..70849 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KB890_RS26450 (70846) 70846..71262 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KB890_RS26455 (71336) 71336..72898 + 1563 WP_004206609.1 AAA family ATPase -
KB890_RS26460 (72883) 72883..73905 + 1023 WP_000361404.1 helicase UvrD -
KB890_RS26465 (74161) 74161..74858 + 698 WP_223174630.1 IS1 family transposase -
KB890_RS26470 (74887) 74887..75159 + 273 Protein_94 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..104361 104361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T199802 WP_001044770.1 NZ_CP073374:70846-71262 [Klebsiella sp. P1954]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T199802 NZ_CP097577:946110-946376 [Streptococcus suis]
TTGAGTTATAAGGTTATTTTGTCTGCTAAGGCTCAAAAGCAAATTCGAAAGATGGATAAAACGGCTGCAAGTTTGATTTT
GCGGTATCTTTACAAGCATATTGACGGTTGCGATAACCCCAGACAATACGGAAAAGCCTTGACTGCTAATCGTTCTGGCC
AATGGCGCTACCGTATCGGCAACTACAGGGTTATCGTCAGTATTGAAGATGACAAATTGATTGTAACGGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT199802 WP_001261282.1 NZ_CP073374:70619-70849 [Klebsiella sp. P1954]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT199802 NZ_CP097577:945881-946120 [Streptococcus suis]
ATGGCAGCCATTACATTGAAAGTTTCTGAGCAAGACAAGCTTTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTGAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGATGAGTACGATGCGCGTGTGGCAGACATTGCTTGGGCAG
AATACCAAGAAGACTTGGCGAATGGAATTGAGCCGATAACATTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References