Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69506..70031 Replicon plasmid p00002
Accession NZ_CP073374
Organism Klebsiella sp. P1954

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KB890_RS26430 Protein ID WP_013023785.1
Coordinates 69506..69811 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KB890_RS26435 Protein ID WP_001568025.1
Coordinates 69813..70031 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB890_RS26400 (65217) 65217..65843 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KB890_RS27635 (65840) 65840..66142 + 303 WP_004197636.1 hypothetical protein -
KB890_RS26410 (66582) 66582..67376 - 795 WP_004197635.1 site-specific integrase -
KB890_RS26415 (67574) 67574..68590 - 1017 WP_017899884.1 hypothetical protein -
KB890_RS26420 (68601) 68601..68915 - 315 WP_053389906.1 hypothetical protein -
KB890_RS26425 (68942) 68942..69337 - 396 WP_017899885.1 hypothetical protein -
KB890_RS26430 (69506) 69506..69811 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KB890_RS26435 (69813) 69813..70031 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KB890_RS26440 (70201) 70201..70662 - 462 WP_160880186.1 hypothetical protein -
KB890_RS26445 (70619) 70619..70849 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
KB890_RS26450 (70846) 70846..71262 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
KB890_RS26455 (71336) 71336..72898 + 1563 WP_004206609.1 AAA family ATPase -
KB890_RS26460 (72883) 72883..73905 + 1023 WP_000361404.1 helicase UvrD -
KB890_RS26465 (74161) 74161..74858 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..104361 104361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T199801 WP_013023785.1 NZ_CP073374:c69811-69506 [Klebsiella sp. P1954]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T199801 NZ_CP097577:568808-568990 [Streptococcus suis]
ATGCCTATGACACAAAAAGAAATGGTTAAGCTGCTTACCGCCCATGGTTGGACAAAGACCAAAGGTGGTAAGGGGTCGCA
TGTTAAACTTGAAAAAGCAGGCGAGCGACCTATAACCATTCCCCATGGTGAAATTAACAAATACACCGAAAGGGGTATCA
AAAAGCAAGCAGGGCTGTTATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT199801 WP_001568025.1 NZ_CP073374:c70031-69813 [Klebsiella sp. P1954]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT199801 NZ_CP097577:569030-569479 [Streptococcus suis]
ATGCTTGTCACTTATCCAGCTTTATTTTATTATGACGATACAGACGGAGCTAACGCCCCTTACTTTGTAACTTTCCCTGA
TTTTGAGCATTCAGCAACTCAGGGCGAAGATATGGCAGACGCTATGGCAATGGCTAGCGATTGGTTAGGCATGAACTTAG
CTGATTATATCGAGAATGGAAGAGACATTCCTACCCCGTCTTCTATAAACACTTTGTCATTAGTAGATAATAACCCCTTC
CGTAATGAGGACTTTGAGATGGTTTACGATTCTAGCAAGTCTTTTATCTCCATGGTGATGGTGGACGTTGCAAAGTATTT
GGGCAGTCAAGAGCCAGTAAAGAAAACACTTACTATCCCACGCTGGGCAGACACTCTAGGACGTGAACTAGGATTGAATT
TCTCACAGACCCTCACGGATGCAATTGCAGATAAAAAAATTCATGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References