Detailed information of TA system TA199791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3335400..3335986
• Replicon: chromosome
• Accession: NZ_CP073373
• Organism: Klebsiella sp. P1954

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KB890_RS15895
• Protein ID: WP_002920800.1
• Coordinates: 3335618..3335986 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: KB890_RS15890
• Protein ID: WP_004174006.1
• Coordinates: 3335400..3335621 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB890_RS15870 (3331557)	3331557..3332483	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KB890_RS15875 (3332480)	3332480..3333757	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
KB890_RS15880 (3333754)	3333754..3334521	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KB890_RS15885 (3334523)	3334523..3335236	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KB890_RS15890 (3335400)	3335400..3335621	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KB890_RS15895 (3335618)	3335618..3335986	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KB890_RS15900 (3336259)	3336259..3337575	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KB890_RS15905 (3337682)	3337682..3338569	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KB890_RS15910 (3338566)	3338566..3339411	+	846	WP_004181473.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KB890_RS15915 (3339413)	3339413..3340483	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3332480..3341220	8740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T199791 WP_002920800.1 NZ_CP073373:3335618-3335986 [Klebsiella sp. P1954]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T199791 NZ_CP097576:c1847674-1847393 [Microcystis aeruginosa str. Chao 1910]
GTGATCCAAAACTTTAAAGATAAAGAAGCTCAAAAAGTTTTTGAACGTAAACATTCGCGTAAATTGCCATTAGATATCCA
ACAAGTGGCATTGCGAAAATTGCGGATGCTTAATCGTGCGGAAACCCTGCAAGATCTTCGTGTCCCACCAGCAAATCGAT
TGGAACGTCTGGTTGGTGACAGGGAAGGTCAATATAGCATTCGGGTTAACGATCAATGGCGAATTTGCTTTGTCTGGCAA
AACGGTGATGCTCTAGACGGAGAAATCGTTGATTATCATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT199791 WP_004174006.1 NZ_CP073373:3335400-3335621 [Klebsiella sp. P1954]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT199791 NZ_CP097576:c1847383-1847069 [Microcystis aeruginosa str. Chao 1910]
ATGAATGAAGATAAACTAATGCCAATTCATCCCGGAGAGGTTCTTTTAGAGGAATTTATCAAGCCAATGAACCTTAGTCA
GAATCAAATAGCTCTGGCTCTTGGAGTTCCCGAACAATGTATTAACGAAATCGTTCACGGAAAGCGGTGTATCACGGCGG
ATATAGCTCTTCGGTTAGCTCGTTATTTTGATATGTCACCGCGATTTTGGCTGGGATTACAAATGGATTACGATCTGGAC
GTTGTTGAAGATGAAATAGGAGATCAATTGGCCAAAGAAGTTGCTGTAATGACAGTTATTTGTGCAGAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7