Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2911390..2912015 Replicon chromosome
Accession NZ_CP073373
Organism Klebsiella sp. P1954

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KB890_RS13930 Protein ID WP_002882817.1
Coordinates 2911390..2911773 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KB890_RS13935 Protein ID WP_004150355.1
Coordinates 2911773..2912015 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB890_RS13915 (2908756) 2908756..2909658 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KB890_RS13920 (2909655) 2909655..2910290 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KB890_RS13925 (2910287) 2910287..2911216 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KB890_RS13930 (2911390) 2911390..2911773 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KB890_RS13935 (2911773) 2911773..2912015 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KB890_RS13940 (2912220) 2912220..2913137 + 918 WP_004181612.1 alpha/beta hydrolase -
KB890_RS13945 (2913151) 2913151..2914092 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KB890_RS13950 (2914137) 2914137..2914574 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KB890_RS13955 (2914571) 2914571..2915431 - 861 WP_004146232.1 virulence factor BrkB family protein -
KB890_RS13960 (2915425) 2915425..2916024 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199790 WP_002882817.1 NZ_CP073373:c2911773-2911390 [Klebsiella sp. P1954]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199790 NZ_CP097576:1841128-1841418 [Microcystis aeruginosa str. Chao 1910]
ATGGCTACTATCCAAACCTATCCTTGGGATGCTGCAGATCATCTGCAAACAAAAGAAGATATAGCGGCATATCTTGAAGC
TGCTCTCGAAGATGGTGATCCGAGTTTAATAATAGCAGCATTAGGTGATATTGCCCGCGCTAAAGGAATAACAAATATTG
CTAGTGAAACAGGGTTAGGAAGTGAAAATCTCTGCAAAGCTTTGTTGAGTGAAGGTAATCCAGAGTTTACTACAGTTATT
AGGATTTTACAGGTACTTGGATTACGTCTCCAGATTGTACCCATTACCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199790 WP_004150355.1 NZ_CP073373:c2912015-2911773 [Klebsiella sp. P1954]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199790 NZ_CP097576:1840828-1841118 [Microcystis aeruginosa str. Chao 1910]
ATGATTGAAATTCGCCAGACTAAAACTTATTCTGAGTGGTTTAGTGGTTTGCGAGATCGCCAAGCAAAAGCGCGTATAGA
CATTCGTATTCGTCGTTTATCTACGGGCAATCCGGGCGATGTTAAGCCAGTAGGACAAGGTGTATCAGAACTGCGAGTTG
ATTATGGTCCAGGCTATCGGGTATATTTTATACAACAAGGTAAGACCTTGATTATCCTCTTGGCAGGAGGAGATAAAAAG
ACTCAAGAACGAGACATCAAAACAGCATTAGATTTAGCCCGTAACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References