Detailed information of TA system TA199782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 106518..107143
• Replicon: plasmid plas2
• Accession: NZ_CP073362
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KDN44_RS25005
• Protein ID: WP_000911313.1
• Coordinates: 106518..106916 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KDN44_RS25010
• Protein ID: WP_000450520.1
• Coordinates: 106916..107143 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS24990 (102843)	102843..103340	+	498	WP_000605857.1	entry exclusion protein	-
KDN44_RS24995 (103372)	103372..104103	+	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
KDN44_RS25000 (104356)	104356..106509	+	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KDN44_RS25005 (106518)	106518..106916	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KDN44_RS25010 (106916)	106916..107143	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KDN44_RS25015 (107225)	107225..111817	+	4593	Protein_132	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aadA5 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..134098	134098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T199782 WP_000911313.1 NZ_CP073362:c106916-106518 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T199782 NZ_CP097575:c2343285-2343007 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT199782 WP_000450520.1 NZ_CP073362:c107143-106916 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT199782 NZ_CP097575:c2342995-2342690 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2