Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 98262..99885 Replicon plasmid plas1-MCR
Accession NZ_CP073361
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KDN44_RS23725 Protein ID WP_000088046.1
Coordinates 98560..99885 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KDN44_RS23720 Protein ID WP_024136327.1
Coordinates 98262..98555 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDN44_RS23695 94134..95375 + 1242 WP_001053338.1 VWA domain-containing protein -
KDN44_RS25935 95466..95597 + 132 WP_000374059.1 hypothetical protein -
KDN44_RS23700 95938..96219 - 282 WP_000077926.1 hypothetical protein -
KDN44_RS23705 96269..96460 - 192 WP_000398480.1 hypothetical protein -
KDN44_RS23710 96552..96893 - 342 WP_001151575.1 hypothetical protein -
KDN44_RS23715 97266..97658 + 393 WP_000341066.1 hypothetical protein -
KDN44_RS23720 98262..98555 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KDN44_RS23725 98560..99885 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KDN44_RS23730 99946..100152 + 207 WP_000134171.1 hypothetical protein -
KDN44_RS23735 100254..100664 + 411 WP_000985911.1 hypothetical protein -
KDN44_RS23740 100677..101492 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KDN44_RS23745 101746..102171 + 426 WP_001043843.1 hypothetical protein -
KDN44_RS23750 102720..103028 + 309 WP_001224687.1 hypothetical protein -
KDN44_RS23755 103044..103901 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid oqxB / oqxA / sul1 / blaCTX-M-65 / mph(A) / tet(M) / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / ant(3'')-Ia / lnu(F) / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / floR / mcr-1.1 - 1..223103 223103


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T199774 WP_000088046.1 NZ_CP073361:98560-99885 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T199774 NZ_CP097572:c1144054-1143836 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT199774 WP_024136327.1 NZ_CP073361:98262-98555 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT199774 NZ_CP097572:c1144456-1144082 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References