Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4395235..4395732 Replicon chromosome
Accession NZ_CP073360
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KDN44_RS21055 Protein ID WP_001173343.1
Coordinates 4395235..4395408 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KDN44_RS21060 Protein ID WP_000398619.1
Coordinates 4395436..4395732 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDN44_RS21045 (4391905) 4391905..4393362 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
KDN44_RS21050 (4393599) 4393599..4395116 + 1518 WP_001295074.1 lysine--tRNA ligase -
KDN44_RS21055 (4395235) 4395235..4395408 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KDN44_RS21060 (4395436) 4395436..4395732 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KDN44_RS21065 (4395959) 4395959..4396231 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KDN44_RS21070 (4396243) 4396243..4396473 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KDN44_RS21075 (4396654) 4396654..4398285 + 1632 WP_001216486.1 sensor histidine kinase -
KDN44_RS21080 (4398282) 4398282..4399001 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T199772 WP_001173343.1 NZ_CP073360:c4395408-4395235 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T199772 NZ_CP097572:c63952-63602 [Enterobacter kobei]
ATGTGGACGGTACTGTTCGGAAAGGTGTTTGAGCTATGGTTGCTTGAACAGGAAGAAGGGTTACAGGAAAAGGTACTGGC
AGACATGCTAAATCTACAAAACTATGGACCTCGTCTTCCTCGTCCTTATGCCGATACTGTAAAAGGCTCGCAATATAACA
ATATGAAAGAGTTACGTATTCAATACGCTGGCCGTCCGATTCGTGCTTTATTCGCATTTGACCCCGAACGTCAGGCGATT
GTGCTTTGCGCAGGCAACAAGAGTAACGACAAGGCATTTTATGAAAAACTCATTCGCATTGCAGATGATGAATTTACCCG
GCATCTGACAAGCCTGGAGACAGCCAAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT199772 WP_000398619.1 NZ_CP073360:c4395732-4395436 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT199772 NZ_CP097572:c63605-63306 [Enterobacter kobei]
GTGAAAACCCTAAATGAATTAATTACATCACGCTCACCGGAAAGCCAACAACGAATTAAAGAAATGGCAGAGGAAATGAT
CCTTGAAACGGGTCTGCAACTTTTACGTGAAGAGCTCAGACTTTCGCAAAAAAATGTTGCCGAGCTAATGGGGGTGAGCC
AGCCCGCAGTAACACAAATAGAGCAACGTGGAAATGAACTAAAAATTGCCACTCTTAAGCGCTACATTGAGGCAATGGGT
GGGAAACTTTGTATTGACGTTGAACTTCCAACAGGAAAACGCGTTGCGTTTCATATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References