Detailed information of TA system TA199770

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4180660..4181495
• Replicon: chromosome
• Accession: NZ_CP073360
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KDN44_RS19985
• Protein ID: WP_212541835.1
• Coordinates: 4180660..4181037 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: KDN44_RS19990
• Protein ID: WP_001285607.1
• Coordinates: 4181127..4181495 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS19950 (4176015)	4176015..4176995	+	981	WP_001366032.1	sialate O-acetylesterase	-
KDN44_RS19955 (4177003)	4177003..4177107	-	105	Protein_3937	HNH endonuclease	-
KDN44_RS19960 (4177207)	4177207..4178079	+	873	WP_000168567.1	HNH endonuclease	-
KDN44_RS19965 (4178190)	4178190..4179188	-	999	WP_001240355.1	membrane protein	-
KDN44_RS19970 (4179727)	4179727..4179873	-	147	WP_212541834.1	hypothetical protein	-
KDN44_RS19975 (4179958)	4179958..4180155	-	198	WP_001327226.1	DUF957 domain-containing protein	-
KDN44_RS19980 (4180175)	4180175..4180663	-	489	WP_000761685.1	DUF5983 family protein	-
KDN44_RS19985 (4180660)	4180660..4181037	-	378	WP_212541835.1	TA system toxin CbtA family protein	Toxin
KDN44_RS19990 (4181127)	4181127..4181495	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KDN44_RS19995 (4181575)	4181575..4181796	-	222	WP_000692350.1	DUF987 domain-containing protein	-
KDN44_RS20000 (4181883)	4181883..4182359	-	477	WP_001384029.1	RadC family protein	-
KDN44_RS20005 (4182374)	4182374..4182859	-	486	WP_000213722.1	antirestriction protein	-
KDN44_RS20010 (4182951)	4182951..4183769	-	819	WP_212541836.1	DUF932 domain-containing protein	-
KDN44_RS20015 (4183859)	4183859..4184068	-	210	WP_212541837.1	DUF905 family protein	-
KDN44_RS20020 (4184098)	4184098..4184775	-	678	WP_160362476.1	hypothetical protein	-
KDN44_RS20025 (4184923)	4184923..4185603	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4172051..4200184	28133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14259.21 Da
• Isoelectric Point: 7.8281

>T199770 WP_212541835.1 NZ_CP073360:c4181037-4180660 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRRLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPWAKQ

Nucleotide

• Length: 378 bp

>T199770 NZ_CP097571:c2516565-2516299 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT199770 WP_001285607.1 NZ_CP073360:c4181495-4181127 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT199770 NZ_CP097571:c2516822-2516565 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1M0JLU8