Detailed information of TA system TA199768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3614041..3614878
• Replicon: chromosome
• Accession: NZ_CP073360
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KDN44_RS17380
• Protein ID: WP_000227784.1
• Coordinates: 3614336..3614878 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: KDN44_RS17375
• Protein ID: WP_024258149.1
• Coordinates: 3614041..3614352 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS17350 (3609061)	3609061..3610008	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KDN44_RS17355 (3610030)	3610030..3612021	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KDN44_RS17360 (3612011)	3612011..3612625	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KDN44_RS17365 (3612625)	3612625..3612954	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KDN44_RS17370 (3612966)	3612966..3613856	+	891	WP_000971336.1	heme o synthase	-
KDN44_RS17375 (3614041)	3614041..3614352	+	312	WP_024258149.1	DUF1778 domain-containing protein	Antitoxin
KDN44_RS17380 (3614336)	3614336..3614878	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KDN44_RS17385 (3614934)	3614934..3615869	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KDN44_RS17390 (3616277)	3616277..3617641	+	1365	WP_023143167.1	MFS transporter	-
KDN44_RS17395 (3617769)	3617769..3618260	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KDN44_RS17400 (3618428)	3618428..3619339	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T199768 WP_000227784.1 NZ_CP073360:3614336-3614878 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T199768 NZ_CP097571:c2459884-2459618 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAGATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11338.99 Da
• Isoelectric Point: 10.2456

>AT199768 WP_024258149.1 NZ_CP073360:3614041-3614352 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQHRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT199768 NZ_CP097571:c2460135-2459884 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BYY