Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 268890..269476 Replicon chromosome
Accession NZ_CP073360
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KDN44_RS01200 Protein ID WP_042014162.1
Coordinates 268890..269120 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KDN44_RS01205 Protein ID WP_000593555.1
Coordinates 269117..269476 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDN44_RS01190 (264927) 264927..267662 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
KDN44_RS01195 (267662) 267662..268786 + 1125 WP_001314210.1 ABC transporter permease -
KDN44_RS01200 (268890) 268890..269120 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KDN44_RS01205 (269117) 269117..269476 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KDN44_RS01210 (269596) 269596..269997 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KDN44_RS01215 (270003) 270003..270809 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KDN44_RS01220 (270806) 270806..271570 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KDN44_RS01225 (271570) 271570..272403 - 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
KDN44_RS01230 (272400) 272400..273344 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T199750 WP_042014162.1 NZ_CP073360:268890-269120 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T199750 NZ_CP097557:c6022267-6021989 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT199750 WP_000593555.1 NZ_CP073360:269117-269476 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT199750 NZ_CP097557:c6021977-6021672 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References