Detailed information of TA system TA199748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 96659..97169
• Replicon: plasmid pSDQ8C180-1
• Accession: NZ_CP073357
• Organism: Proteus terrae subsp. cibarius strain SDQ8C180-2T

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KDN45_RS18605
• Protein ID: WP_019281448.1
• Coordinates: 96659..96922 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7X5B7D9
• Locus tag: KDN45_RS18610
• Protein ID: WP_009836585.1
• Coordinates: 96915..97169 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN45_RS18570 (KDN45_18480)	91680..92429	+	750	WP_071547986.1	signal peptidase I	-
KDN45_RS18575 (KDN45_18485)	92509..92766	+	258	WP_159242425.1	hypothetical protein	-
KDN45_RS18580 (KDN45_18490)	92753..93178	+	426	WP_206086776.1	hypothetical protein	-
KDN45_RS18590 (KDN45_18500)	94322..94732	-	411	WP_206086781.1	IS200/IS605 family transposase	-
KDN45_RS18595 (KDN45_18505)	94787..95992	+	1206	WP_223715756.1	RNA-guided endonuclease TnpB family protein	-
KDN45_RS18600 (KDN45_18510)	96036..96380	+	345	Protein_112	transposase	-
KDN45_RS18605 (KDN45_18515)	96659..96922	-	264	WP_019281448.1	Txe/YoeB family addiction module toxin	Toxin
KDN45_RS18610 (KDN45_18520)	96915..97169	-	255	WP_009836585.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KDN45_RS18615 (KDN45_18525)	97530..98504	+	975	WP_223674028.1	IS5-like element IS5 family transposase	-
KDN45_RS18620 (KDN45_18530)	98649..98816	-	168	Protein_116	hypothetical protein	-
KDN45_RS18625 (KDN45_18535)	98833..99996	-	1164	WP_016243884.1	1,3-propanediol dehydrogenase	-
KDN45_RS18630 (KDN45_18540)	100141..100433	-	293	Protein_118	hypothetical protein	-
KDN45_RS18635 (KDN45_18545)	100530..100850	+	321	WP_206086774.1	IS66 family insertion sequence element accessory protein TnpB	-
KDN45_RS18645 (KDN45_18555)	101060..101221	-	162	Protein_120	transposase	-
KDN45_RS18650 (KDN45_18560)	101250..101582	-	333	WP_125894547.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / msr(E) / mph(E) / tet(D) / aac(3)-IId / aph(3')-Ia / sul1 / blaDHA-1 / qacE / aadA2 / dfrA12 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / ant(3'')-Ia / dfrA1 / lnu(F)	gmd / fcl / manC / ugd	1..192359	192359
	-	inside	IScluster/Tn	-	-	93468..101119	7651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10330.69 Da
• Isoelectric Point: 8.8578

>T199748 WP_019281448.1 NZ_CP073357:c96922-96659 [Proteus terrae subsp. cibarius]
MSSRLLSWTDESWNDYVYWQTQDKKTLKRINKLITDVKRSPFEGIGKPEPLKENLSGFWSRRIDDANRLVYAVDDSAITI
ISCRYHY

Nucleotide

• Length: 264 bp

>T199748 NZ_CP097557:4172100-4172678 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCACTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGATAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9462.65 Da
• Isoelectric Point: 5.5802

>AT199748 WP_009836585.1 NZ_CP073357:c97169-96915 [Proteus terrae subsp. cibarius]
MRIVSFTEARNSLKSVLDAVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLHRSIAQFKAGKVSQRD
LIDE

Nucleotide

• Length: 255 bp

>AT199748 NZ_CP097557:4171457-4172011 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCCCGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X5B7D9