Detailed information of TA system TA199747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 10087..10664
• Replicon: plasmid pSDQ8C180-1
• Accession: NZ_CP073357
• Organism: Proteus terrae subsp. cibarius strain SDQ8C180-2T

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: KDN45_RS18095
• Protein ID: WP_023159957.1
• Coordinates: 10087..10419 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KDN45_RS18100
• Protein ID: WP_096864992.1
• Coordinates: 10419..10664 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN45_RS19200	5819..5953	-	135	WP_256326490.1	hypothetical protein	-
KDN45_RS18065 (KDN45_17970)	5969..6922	-	954	WP_048821757.1	site-specific integrase	-
KDN45_RS18070 (KDN45_17975)	7352..8049	+	698	WP_211838731.1	IS1 family transposase	-
KDN45_RS18075 (KDN45_17980)	8318..8572	+	255	WP_135017207.1	hypothetical protein	-
KDN45_RS18080 (KDN45_17985)	8668..9126	+	459	WP_223674058.1	hypothetical protein	-
KDN45_RS18085 (KDN45_17990)	9446..9589	+	144	WP_223674057.1	Hok/Gef family protein	-
KDN45_RS18090 (KDN45_17995)	9676..10056	-	381	WP_096864991.1	hypothetical protein	-
KDN45_RS18095 (KDN45_18000)	10087..10419	-	333	WP_023159957.1	endoribonuclease MazF	Toxin
KDN45_RS18100 (KDN45_18005)	10419..10664	-	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KDN45_RS18105 (KDN45_18010)	10950..11573	+	624	WP_023159958.1	AAA family ATPase	-
KDN45_RS18110 (KDN45_18015)	11682..11909	+	228	WP_096864993.1	plasmid partition protein ParG	-
KDN45_RS18115 (KDN45_18020)	12007..12375	-	369	WP_223674056.1	hypothetical protein	-
KDN45_RS18120 (KDN45_18025)	12890..13258	+	369	WP_223674055.1	hypothetical protein	-
KDN45_RS18125 (KDN45_18030)	13261..14253	-	993	WP_223715755.1	IS630 family transposase	-
KDN45_RS18130 (KDN45_18035)	14268..15392	-	1125	WP_143970431.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / msr(E) / mph(E) / tet(D) / aac(3)-IId / aph(3')-Ia / sul1 / blaDHA-1 / qacE / aadA2 / dfrA12 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / ant(3'')-Ia / dfrA1 / lnu(F)	gmd / fcl / manC / ugd	1..192359	192359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T199747 WP_023159957.1 NZ_CP073357:c10419-10087 [Proteus terrae subsp. cibarius]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T199747 NZ_CP097557:2209951-2210538 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT199747 WP_096864992.1 NZ_CP073357:c10664-10419 [Proteus terrae subsp. cibarius]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT199747 NZ_CP097557:2210605-2211459 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75