Detailed information of TA system TA199740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1032..1506
• Replicon: plasmid unnamed2
• Accession: NZ_CP073353
• Organism: Klebsiella pneumoniae strain CP19

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: KBT94_RS27620
• Protein ID: WP_004197762.1
• Coordinates: 1032..1298 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: KBT94_RS27625
• Protein ID: WP_000879771.1
• Coordinates: 1282..1506 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBT94_RS27610	1..546	+	546	WP_023317533.1	hypothetical protein	-
KBT94_RS27615	612..962	+	351	WP_004197756.1	hypothetical protein	-
KBT94_RS27620	1032..1298	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBT94_RS27625	1282..1506	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
KBT94_RS27630	2173..2385	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
KBT94_RS27635	2392..2901	-	510	WP_032433972.1	MbeB family mobilization protein	-
KBT94_RS27640	2936..3370	-	435	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
KBT94_RS27645	3579..3923	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
KBT94_RS27650	3913..4089	-	177	WP_004197757.1	hypothetical protein	-
KBT94_RS27655	5246..5572	+	327	WP_050516389.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5597	5597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T199740 WP_004197762.1 NZ_CP073353:c1298-1032 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T199740 NZ_CP097556:c143529-143248 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT199740 WP_000879771.1 NZ_CP073353:c1506-1282 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT199740 NZ_CP097556:c143753-143526 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5