Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 48263..48906 Replicon plasmid unnamed1
Accession NZ_CP073352
Organism Klebsiella pneumoniae strain CP19

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KBT94_RS27390 Protein ID WP_001044770.1
Coordinates 48490..48906 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KBT94_RS27385 Protein ID WP_001261282.1
Coordinates 48263..48493 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBT94_RS27350 43486..44265 - 780 WP_013214009.1 site-specific integrase -
KBT94_RS27355 44449..45453 - 1005 WP_011977814.1 hypothetical protein -
KBT94_RS27360 45483..45686 - 204 WP_011977813.1 hypothetical protein -
KBT94_RS27365 45732..46253 - 522 WP_013214008.1 hypothetical protein -
KBT94_RS27370 46311..46703 - 393 WP_011977811.1 hypothetical protein -
KBT94_RS27375 46740..47684 - 945 WP_011977810.1 hypothetical protein -
KBT94_RS27380 47845..48306 - 462 WP_014343465.1 hypothetical protein -
KBT94_RS27385 48263..48493 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KBT94_RS27390 48490..48906 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KBT94_RS27395 48980..50542 + 1563 WP_004206609.1 AAA family ATPase -
KBT94_RS27400 50527..51549 + 1023 WP_000361404.1 helicase UvrD -
KBT94_RS27405 51805..52502 + 698 WP_223174630.1 IS1 family transposase -
KBT94_RS27410 52531..52803 + 273 Protein_75 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaCTX-M-65 - 1..80922 80922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T199739 WP_001044770.1 NZ_CP073352:48490-48906 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T199739 NZ_CP097555:c5321063-5320785 [Pseudomonas aeruginosa]
GTGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT199739 WP_001261282.1 NZ_CP073352:48263-48493 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT199739 NZ_CP097555:c5320773-5320468 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References