Detailed information of TA system TA199720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE(toxin)
• Location: 4484902..4485430
• Replicon: chromosome
• Accession: NZ_CP073347
• Organism: Marinobacterium rhizophilum strain D13-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KDW95_RS20230
• Protein ID: WP_255853571.1
• Coordinates: 4485149..4485430 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KDW95_RS20225
• Protein ID: WP_255853570.1
• Coordinates: 4484902..4485159 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDW95_RS20190 (KDW95_20085)	4480004..4480315	+	312	WP_255853565.1	hypothetical protein	-
KDW95_RS20195 (KDW95_20090)	4481168..4481377	+	210	WP_255853566.1	hypothetical protein	-
KDW95_RS20200 (KDW95_20095)	4481710..4482144	+	435	WP_255853567.1	IS200/IS605 family transposase	-
KDW95_RS20205 (KDW95_20100)	4482236..4482562	+	327	WP_255853568.1	hypothetical protein	-
KDW95_RS20210	4483108..4483245	+	138	Protein_3948	RHS repeat-associated core domain-containing protein	-
KDW95_RS20215 (KDW95_20110)	4483740..4484162	+	423	WP_255853569.1	hypothetical protein	-
KDW95_RS20220 (KDW95_20115)	4484659..4484832	-	174	WP_255856571.1	hypothetical protein	-
KDW95_RS20225 (KDW95_20120)	4484902..4485159	+	258	WP_255853570.1	antitoxin	Antitoxin
KDW95_RS20230 (KDW95_20125)	4485149..4485430	+	282	WP_255853571.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDW95_RS20235 (KDW95_20130)	4485545..4485889	+	345	Protein_3953	hypothetical protein	-
KDW95_RS20240 (KDW95_20135)	4485886..4486212	+	327	WP_255853572.1	hypothetical protein	-
KDW95_RS20245 (KDW95_20140)	4486954..4488288	+	1335	WP_255853573.1	group II intron reverse transcriptase/maturase	-
KDW95_RS20250 (KDW95_20145)	4488960..4490267	-	1308	WP_255853574.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4481710..4482144	434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11162.97 Da
• Isoelectric Point: 10.2813

>T199720 WP_255853571.1 NZ_CP073347:4485149-4485430 [Marinobacterium rhizophilum]
MTYRLKFHPDALEEWERLPSAVKEYFKGKLRERLEHPHVPKARLSGGYNLYKIKRKHPPFRLTYHVNDKELIVTALSVGK
RDGDVYREMLERL

Nucleotide

• Length: 282 bp

>T199720 NZ_CP097529:c4750613-4750317 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9198.54 Da
• Isoelectric Point: 4.0610

>AT199720 WP_255853570.1 NZ_CP073347:4484902-4485159 [Marinobacterium rhizophilum]
MSVERMMAGISASITDFKANPNAIIQRSGGEAVAVLKSNVPCFYAVPPELFEMMSEAVEDLALLTQARERLGDGKEPVEV
NIDDL

Nucleotide

• Length: 258 bp

>AT199720 NZ_CP097529:c4750314-4749913 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA