Detailed information of TA system TA199719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 987336..988532
• Replicon: chromosome
• Accession: NZ_CP073346
• Organism: Pseudomonas benzenivorans strain D13-4

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KDW96_RS04565
• Protein ID: WP_255839244.1
• Coordinates: 987336..988088 (-)
• Length: 251 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KDW96_RS04570
• Protein ID: WP_255839245.1
• Coordinates: 988164..988532 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDW96_RS04550 (KDW96_04525)	983599..984855	+	1257	WP_255839242.1	MgtC/SapB family protein	-
KDW96_RS04555 (KDW96_04530)	984861..986225	-	1365	WP_255840474.1	DNA recombination protein RmuC	-
KDW96_RS04560 (KDW96_04535)	986413..986820	-	408	WP_255839243.1	sel1 repeat family protein	-
KDW96_RS04565 (KDW96_04540)	987336..988088	-	753	WP_255839244.1	RES family NAD+ phosphorylase	Toxin
KDW96_RS04570 (KDW96_04545)	988164..988532	-	369	WP_255839245.1	DUF2384 domain-containing protein	Antitoxin
KDW96_RS04575 (KDW96_04550)	988671..991280	+	2610	WP_255839246.1	YdbH domain-containing protein	-
KDW96_RS04580 (KDW96_04555)	991294..991485	+	192	WP_255839247.1	YnbE family lipoprotein	-
KDW96_RS04585 (KDW96_04560)	991497..991841	+	345	WP_255839248.1	YdbL family protein	-
KDW96_RS04595 (KDW96_04570)	992334..993203	-	870	WP_255839249.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (62-206)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 251 a.a.
• Molecular weight: 28113.16 Da
• Isoelectric Point: 8.6274

>T199719 WP_255839244.1 NZ_CP073346:c988088-987336 [Pseudomonas benzenivorans]
MDIWQACRGVEQVAPLRGRLVRLVESQEQVATLQLVDNLAEQALLEELLESSKPPLPRDAEPLHYLLKTPFRYPPLRWGS
RFGTRQEPSLFYAALRLETAMAEAAYYRFVLWDGMASAPPSGRVLSEHASFEARYQVARGIRLQRPPFSRFQRALSHPSD
YRGTQALGASMRAAGVEAFEYRSARCPLGGSNVALYAPGALAEKRPRNLLPWLCETTPGYVAFKHAQAPDTPRMFHFEDY
LVDGQLPLPA

Nucleotide

• Length: 753 bp

>T199719 NZ_CP097529:4691199-4691774 [Pseudomonas putida]
ATGAAGCACTCGCCCTTCACGGCCATATACGACGCGAATGTACTTTACCCTGCTCCACTGCGTGATTTCCTGATGAACCT
AGCGCTGACGGGCATCTACCGGGCACGTTGGTCAGCGAGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGAC
CTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTAC
GATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTC
GGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACG
ACTTCATCGCTGACCTTTGGGACCTGGACAAGGCAGCAGTCCTCGAAGCTGCCCAGCGGCAGCGAATATCGCTGAAGAAC
CCACCGCACAGCGTACAGCAGTATCTCGACAGGCTGCTTCAGCAGAAGCTTCCCGAGACAGTGAAACTGCTTTCAGCATT
CAAGTTCCTCCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13501.73 Da
• Isoelectric Point: 10.6605

>AT199719 WP_255839245.1 NZ_CP073346:c988532-988164 [Pseudomonas benzenivorans]
MSALARPHADADAVLAKALLNTREQLGLTQQELADIVGVHRTAISRWADSGLRPQSKTGELALLLIRVYRALFALFGGNL
QDMRHFLRTENRHLAGVPLALMRQVQGLVHVVEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT199719 NZ_CP097529:4690735-4691202 [Pseudomonas putida]
ATGACCACCGCCAACCTCTCTAGCCATGTGCTTCCTGACGAGAAAGAAATTGCCGTGGCAGTCGAGTCAAGCCGCACGCT
GGCAGCGTTCCTCTCGACAAAATCAGATACCCAACGGATCGAACTGCTCAACGAGGAAAATCAGCGCCAGGTTGTTGAAG
TACCTACTTTCGCCCTACGCCTATTCGGCGAAATTCTCAGCGAACTGGCCTTGGGTAACTCAGTCAAAGTCGTTCCAATC
CACGCCGAACTAACTACCCAGGAAGCAGCAGACCTGCTTAACGTCTCCCGGCCGCACTTGGTAAAGCTGCTCGACGAAGC
GGTGATTCCGCACACCAAGACAGGCCGCCATCGTCGTGTCAAATTCTCAGACCTGGTTGCCTACAAGGACAAACGTGATG
GGGTGAGCCGCGCTGCGATAGATGCGCTGGCAGCCCAAGCTCAGGAATTGAAGATGGGGTACGAATGA