Detailed information of TA system TA199718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /PRK10151(toxin)
• Location: 978347..979759
• Replicon: chromosome
• Accession: NZ_CP073346
• Organism: Pseudomonas benzenivorans strain D13-4

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KDW96_RS04540
• Protein ID: WP_255839240.1
• Coordinates: 979190..979759 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KDW96_RS04535
• Protein ID: WP_255839239.1
• Coordinates: 978347..979186 (-)
• Length: 280 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDW96_RS04510 (KDW96_04485)	973644..974564	+	921	WP_255839234.1	recombination-associated protein RdgC	-
KDW96_RS04515 (KDW96_04490)	974716..975030	+	315	WP_255839235.1	quaternary ammonium compound efflux SMR transporter SugE	-
KDW96_RS04520 (KDW96_04495)	975085..976962	-	1878	WP_255839236.1	MFS transporter	-
KDW96_RS04525 (KDW96_04500)	977068..977397	-	330	WP_255839237.1	AzlD domain-containing protein	-
KDW96_RS04530 (KDW96_04505)	977397..978110	-	714	WP_255839238.1	AzlC family ABC transporter permease	-
KDW96_RS04535 (KDW96_04510)	978347..979186	-	840	WP_255839239.1	AraC family transcriptional regulator	Antitoxin
KDW96_RS04540 (KDW96_04515)	979190..979759	-	570	WP_255839240.1	GNAT family protein	Toxin
KDW96_RS04545 (KDW96_04520)	980005..983487	+	3483	WP_255839241.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (198-274)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21664.10 Da
• Isoelectric Point: 11.1703

>T199718 WP_255839240.1 NZ_CP073346:c979759-979190 [Pseudomonas benzenivorans]
VFKPQLVTLQRGALRIEPMVDADIPPLVSLAEANRQELIYMSGAQRLDWYRQGLLQQREGKALPFVIRLAERLVGTTRFG
DFMPTLPAAEIGWTWLDRSQHGSGLNSMIKFLMLRHAFESWRMVRVQLKTAASNLRAQRAIEKLGAQREGLLRNHRRLAD
GRLDDSVLYSVTDQDWPAIKRRLEARFGA

Nucleotide

• Length: 570 bp

>T199718 NZ_CP097529:c4415508-4415260 [Pseudomonas putida]
ATGTCTAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCTGAGCTGGTCACGCTGCT
GGGTGGGCTTGGATACCGCCATATCGAGGGTGATGGCAGCCGAGTGAAGTTCGACAATGGCCACCCGCAGGCAATGATCA
ACTTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTAATAGAGCACCTGAAAGCAGGAGGA
TTGATCTGA

Antitoxin

Protein

• Length: 280 a.a.
• Molecular weight: 31314.91 Da
• Isoelectric Point: 9.4261

>AT199718 WP_255839239.1 NZ_CP073346:c979186-978347 [Pseudomonas benzenivorans]
MNRLSTHEQVRFSTAGDIGDLELLSARFIEHRFAPHVHDGYVIAVLEAGAERYRYRGEEHLANAGSIALINPDEVHTGAK
GCDQGWRYRVFYPHTRQIQVLLEELELPGTGLPMFRGSVHRDADLVASLGELHRLLENDQATLLQRQTHWREAMLQLLLR
HARLPQPSRAGCEPRAVARAKELLASRLPEPPSLEEMALAVNLSPFHFARVFRRATGLPPHAWLKLRRLEQARALLKSGC
TPLAVAIQLGFADQSHLNRQFKQAYGVAPGAYRRACLGA

Nucleotide

• Length: 840 bp

>AT199718 NZ_CP097529:c4415260-4414919 [Pseudomonas putida]
ATGAGCACCATGTTGCAGTACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAGGACAATTGCCTATTCGGCAAGCT
TCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCTGAACTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTATCTCGACACCTGCGCATCACTCGGGCAGAACCCGGAAATTCCGTGCAAGGGGTCGTTCAATGTGAGGGTAGGGCAC
GACCTGCACCTAGCGGCCAGCGTGGCAGCCACCAAGCAAAGTATTTCCTTGAATGATCTGACACGCAAAGCGCTTAGTGA
GTACCTGGGTCATCACGCGTAG