Detailed information of TA system TA199712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2243859..2244510
• Replicon: chromosome
• Accession: NZ_CP073317
• Organism: Lacticaseibacillus rhamnosus strain CE1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: KAW33_RS10420
• Protein ID: WP_005686631.1
• Coordinates: 2243859..2244242 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: KAW33_RS10425
• Protein ID: WP_005686632.1
• Coordinates: 2244262..2244510 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAW33_RS10415 (KAW33_10415)	2242895..2243422	-	528	WP_005711058.1	QueT transporter family protein	-
KAW33_RS10420 (KAW33_10420)	2243859..2244242	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAW33_RS10425 (KAW33_10425)	2244262..2244510	-	249	WP_005686632.1	antitoxin	Antitoxin
KAW33_RS10430 (KAW33_10430)	2244623..2245762	-	1140	WP_005691215.1	alanine racemase	-
KAW33_RS10435 (KAW33_10435)	2245749..2246123	-	375	WP_005686634.1	holo-ACP synthase	-
KAW33_RS10440 (KAW33_10440)	2246292..2247800	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
KAW33_RS10445 (KAW33_10445)	2248074..2249462	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T199712 WP_005686631.1 NZ_CP073317:c2244242-2243859 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T199712 NZ_CP097529:332741-333217 [Pseudomonas putida]
TTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATATCTGGAT
CGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGA
TTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCTGACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCT
TTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGAATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGC
CAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGC
GAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT199712 WP_005686632.1 NZ_CP073317:c2244510-2244262 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT199712 NZ_CP097529:333259-333732 [Pseudomonas putida]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19