199709

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5164952..5165691 Replicon chromosome
Accession NZ_CP073310
Organism Enterobacter cloacae strain CZ862

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag KDU74_RS25480 Protein ID WP_013087172.1
Coordinates 5165206..5165691 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag KDU74_RS25475 Protein ID WP_012540086.1
Coordinates 5164952..5165218 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDU74_RS25445 (KDU74_25445) 5160182..5160718 - 537 WP_178328939.1 GNAT family N-acetyltransferase -
KDU74_RS25450 (KDU74_25450) 5160843..5162600 - 1758 WP_218620135.1 arsenical pump-driving ATPase -
KDU74_RS25455 (KDU74_25455) 5162621..5162983 - 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
KDU74_RS25460 (KDU74_25460) 5163059..5163604 - 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -
KDU74_RS25465 (KDU74_25465) 5163613..5164326 - 714 WP_178328938.1 arsenical resistance protein ArsH -
KDU74_RS25470 (KDU74_25470) 5164328..5164651 - 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
KDU74_RS25475 (KDU74_25475) 5164952..5165218 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
KDU74_RS25480 (KDU74_25480) 5165206..5165691 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
KDU74_RS25485 (KDU74_25485) 5166067..5166764 + 698 WP_225092987.1 IS1-like element IS1X4 family transposase -
KDU74_RS25490 (KDU74_25490) 5167127..5168035 - 909 WP_001372261.1 HNH endonuclease -
KDU74_RS25495 (KDU74_25495) 5168290..5169270 - 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -
KDU74_RS25500 (KDU74_25500) 5169595..5170563 - 969 WP_074181092.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage sul2 gtrB / gtrA / fliC / fliA 5092052..5201861 109809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T199709 WP_013087172.1 NZ_CP073310:5165206-5165691 [Enterobacter cloacae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T199709 NZ_CP097528:c4415662-4415414 [Pseudomonas putida]
ATGTCTAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCTGAGCTGGTCACGCTGCT
GGGTGGGCTTGGATACCGCCATATCGAGGGTGATGGCAGCCGAGTGAAGTTCGACAATGGCCACCCGCAGGCAATGATCA
ACTTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTAATAGAGCACCTGAAAGCAGGAGGA
TTGATCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT199709 WP_012540086.1 NZ_CP073310:5164952-5165218 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT199709 NZ_CP097528:c4415414-4415073 [Pseudomonas putida]
ATGAGCACCATGTTGCAGTACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAGGACAATTGCCTATTCGGCAAGCT
TCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCTGAACTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTATCTCGACACCTGCGCATCACTCGGGCAGAACCCGGAAATTCCGTGCAAGGGGTCGTTCAATGTGAGGGTAGGGCAC
GACCTGCACCTAGCGGCCAGCGTGGCAGCCACCAAGCAAAGTATTTCCTTGAATGATCTGACACGCAAAGCGCTTAGTGA
GTACCTGGGTCATCACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References